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Titolo:
DIFFERENTIAL S100-BETA EXPRESSION IN CHOROIDAL AND SKIN MELANOMAS - QUANTITATION BY THE POLYMERASE CHAIN-REACTION
Autore:
KANMITCHELL J; LIGGETT PE; TAYLOR CR; RAO N; GRANADA ESV; DANENBERG KD; WHITE WL; VANELDIK LJ; HORIKOSHI T; DANENBERG PV;
Indirizzi:
UNIV SO CALIF,SCH MED,DEPT PATHOL,2025 ZONAL AVE LOS ANGELES CA 90033 WAKE FOREST UNIV,BOWMAN GRAY SCH MED,DEPT PATHOL WINSTON SALEM NC 27103 UNIV SO CALIF,SCH MED,DEPT OPHTHALMOL LOS ANGELES CA 90033 UNIV SO CALIF,SCH MED,DEPT BIOCHEM LOS ANGELES CA 90033 VANDERBILT UNIV,DEPT PHARMACOL & CELL BIOL NASHVILLE TN 37240
Titolo Testata:
Investigative ophthalmology & visual science
fascicolo: 12, volume: 34, anno: 1993,
pagine: 3366 - 3375
SICI:
0146-0404(1993)34:12<3366:DSEICA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-SEQUENCE; UVEAL MELANOMA; S-100 PROTEIN; MONOCLONAL-ANTIBODIES; S100 PROTEIN; MALIGNANT MELANOMAS; CUTANEOUS MELANOMA; CELL-PROLIFERATION; ANTIGENIC PROFILE; MESSENGER-RNA;
Keywords:
S100 MESSENGER RNA; SKIN MELANOMA; OCULAR MELANOMA; QUANTITATION; POLYMERASE CHAIN REACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
J. Kanmitchell et al., "DIFFERENTIAL S100-BETA EXPRESSION IN CHOROIDAL AND SKIN MELANOMAS - QUANTITATION BY THE POLYMERASE CHAIN-REACTION", Investigative ophthalmology & visual science, 34(12), 1993, pp. 3366-3375

Abstract

Purpose. S100beta, a member of a calcium-binding protein family (S100s), is an important clinical marker for skin melanoma. In contrast, uveal melanomas appeared to express S100beta protein less frequently andto a lesser degree. This study was performed to verify and extend this finding to the mRNA level. Methods. A quantitative polymerase chain reaction (PCR)-based method was used. A ratio, comparing the S100beta PCR fragment to that of beta-actin (an internal reference gene), was generated to compare S100beta mRNA expression among samples. Results. The ratios for skin melanomas (1.2 to 3.9; three tissues and two cell lines) were significantly higher than that for choroidal melanomas (0.1to 0.63; seven of eight primary tumors and four of four cell lines). Only one choroidal melanoma biopsy had a ratio greater than 1. The PCRproducts from choroidal melanoma were identical in size and sequence to the S100beta, as determined by gel electrophoresis and RNA conformational polymorphism. Because the ratios were also low in choroidal melanoma cell lines, the S100beta phenotype appears to be genetically stable. Conclusion. S100beta is differentially expressed at the RNA and protein levels by skin and choroidal melanomas, which are derived from distinct populations of melanocytes. However, choroidal melanomas expressing little or no S100beta were significantly stained by antiserum specific for the S100 protein family. Taken together, these data suggest that choroidal melanocytes express another, perhaps even novel, S100protein(s).

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Documento generato il 28/11/20 alle ore 09:55:45