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Titolo:
IL-1 MESSENGER-RNA EXPRESSION DETECTED BY IN-SITU HYBRIDIZATION AND POLYMERASE CHAIN-REACTION IN HUMAN THYMUS AND THYMOMA - CORRELATION WITH LATE T-CELL MATURATION IN NORMAL VERSUS NEOPLASTIC THYMUS
Autore:
AIME C; BRISTOL L; COHENKAMINSKY S; BERRIHAKNIN S; DURUM S; TAKACS L;
Indirizzi:
AMGEN INC,AMGEN CTR BLDG 8,1840 DEHAVILLAND DR THOUSAND OAKS CA 91320 FAC MED PARIS SUD,DEPT HUMAN PHYSIOL,CELLULAR IMMUNOL LAB,CNRS,URA D1159 F-92350 LE PLESSIS ROBINS FRANCE NIAAA,SPECIAL PROJECTS UNIT,PHYSIOL & PHARMACOL STUDIES LAB ROCKVILLEMD 20852 NCI,BIOL RESPONSE MODIFIERS PROGRAM,MOLEC IMMUNOREGULAT LAB FREDERICKMD 21701
Titolo Testata:
Thymus
fascicolo: 1, volume: 22, anno: 1993,
pagine: 45 - 54
SICI:
0165-6090(1993)22:1<45:IMEDBI>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORTICAL THYMOCYTE; GENE-EXPRESSION; INTERLEUKIN-1; INDUCTION; RECEPTOR; DELETION; DIFFERENTIATION; LYMPHOCYTE; ANTIBODIES; PHENOTYPE;
Keywords:
IN-SITU HYBRIDIZATION; INTERLEUKIN-1; THYMOMAS; THYMUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
C. Aime et al., "IL-1 MESSENGER-RNA EXPRESSION DETECTED BY IN-SITU HYBRIDIZATION AND POLYMERASE CHAIN-REACTION IN HUMAN THYMUS AND THYMOMA - CORRELATION WITH LATE T-CELL MATURATION IN NORMAL VERSUS NEOPLASTIC THYMUS", Thymus, 22(1), 1993, pp. 45-54

Abstract

Interleukin-1 has long been suggested to participate in the development of T-cells in the thymus. To determine whether IL-1 was produced inthe human thymus, we used the technique of in situ hybridization and PCR amplification of mRNA, High levels of IL-1 mRNA were detected in cells at the cortico-medullary border, which separates immature from mature T-cells, suggesting a role for IL-1 in a late stage in T-cell maturation. IL-1 transcripts were detectable by a single step PCR reaction in these samples. We examined a series thymomas (human thymic epithelial tumors) that induce partial T-cell maturation: In this group of thymomas T-cells develop to the cortical stage but not beyond. We failed to detect high levels of IL-1 mRNA expression in these tumors by in-situ hybridization, IL-1 mRNA was detected only in one of these samples by a single step PCR amplification. However, alt of the samples werepositive, when analyzed with a more sensitive, two step amplificationof IL-1 mRNA by the use of nested primers. These results are consistent with the hypothesis that higher level of IL-1 expression is either the consequence of, or required for late T-cell maturation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 20:07:36