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Titolo:
BASIC FIBROBLAST GROWTH-FACTOR MODULATES INTEGRIN EXPRESSION IN MICROVASCULAR ENDOTHELIAL-CELLS
Autore:
KLEIN S; GIANCOTTI FG; PRESTA M; ALBELDA SM; BUCK CA; RIFKIN DB;
Indirizzi:
NYU MED CTR,RAYMOND & BEVERLY SACKLER FDN LAB,DEPT CELL BIOL NEW YORKNY 10016 NYU MED CTR,RAYMOND & BEVERLY SACKLER FDN LAB,DEPT PATHOL NEW YORK NY10016 WISTAR INST ANAT & BIOL PHILADELPHIA PA 19104
Titolo Testata:
Molecular biology of the cell
fascicolo: 10, volume: 4, anno: 1993,
pagine: 973 - 982
SICI:
1059-1524(1993)4:10<973:BFGMIE>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR PRODUCTION; NECROSIS-FACTOR-ALPHA; EXTRACELLULAR-MATRIX; DNA-SYNTHESIS; ADHESION RECEPTORS; ANGIOGENESIS; SUBUNIT; VITRONECTIN; MIGRATION; PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
S. Klein et al., "BASIC FIBROBLAST GROWTH-FACTOR MODULATES INTEGRIN EXPRESSION IN MICROVASCULAR ENDOTHELIAL-CELLS", Molecular biology of the cell, 4(10), 1993, pp. 973-982

Abstract

During angiogenesis capillary endothelial cells undergo a coordinatedset of modifications in their interactions with extracellular matrix components. In this study we have investigated the effect of the prototypical angiogenic factor basic fibroblast growth factor (bFGF) on theexpression and function of several integrins in microvascular endothelial cells. Immunoprecipitation experiments with antibodies to individual subunits indicated that microvascular cells express at their surface several integrins. These include the alpha 1 beta 1, alpha 2 beta 1, and alpha 3 beta 1 laminin/collagen receptors; the alpha 6 beta 1 laminin receptor; the alpha 5 beta 1 and alpha v beta 1 fibronectin receptors; the alpha 6 beta 4 basement membrane receptor; and the alpha v beta 3 and alpha v beta 5 vitronectin receptors. Treatment with bFGF caused a significant increase in the surface expression of the alpha 2 beta 1, alpha 3 beta 1, alpha 5 beta 1, alpha 6 beta 1, alpha 6 beta 4, and alpha v beta 5 integrins. In contrast, the level of expression of the alpha 1 beta 1 and alpha v beta 3 integrins was decreased in bFGF-treated cells. Immunoprecipitation of metabolically labeled cells indicated that bFGF increases the biosynthesis of the alpha 3, alpha 5, alpha 6, beta 4, and beta 5 subunits and decreases the production of the alpha v and beta 3 subunits. These results suggest that bFGF modulates integrin expression by altering the biosynthesis of individual alpha or beta subunits. In accordance with the upregulation of several integrins observed in bFGF-treated cells, these cells adhered better to fibronectin, laminin, vitronectin, and type I collagen than did untreated cells. The largest differences in beta 1 integrin expression occurred similar to 72 h after exposure to bFGF, at a time when the expression of the endothelial cell-to-cell adhesion molecule endoCAM was alsosignificantly upregulated. In contrast, a shorter exposure to bFGF (24-48 h) was required for the maximal induction of plasminogen activator production in the same cells. Taken together, these results show that bFGF causes significant changes in the level of expression and function of several integrins in microvascular endothelial cells.

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Documento generato il 28/11/20 alle ore 23:38:34