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Titolo:
DROSOPHILA NERVOUS-SYSTEM MUSCARINIC ACETYLCHOLINE-RECEPTOR - TRANSIENT FUNCTIONAL EXPRESSION AND LOCALIZATION BY IMMUNOCYTOCHEMISTRY
Autore:
BLAKE AD; ANTHONY NM; CHEN HH; HARRISON JB; NATHANSON NM; SATTELLE DB;
Indirizzi:
UNIV CAMBRIDGE,DEPT ZOOL,MOLEC SIGNALLING LAB,AFRC,DOWNING ST CAMBRIDGE CB2 3EJ ENGLAND UNIV CAMBRIDGE,DEPT ZOOL,MOLEC SIGNALLING LAB,AFRC,DOWNING ST CAMBRIDGE CB2 3EJ ENGLAND UNIV WASHINGTON,SCH MED,DEPT PHARMACOL SEATTLE WA 98195
Titolo Testata:
Molecular pharmacology
fascicolo: 4, volume: 44, anno: 1993,
pagine: 716 - 724
SICI:
0026-895X(1993)44:4<716:DNMA-T>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
COCKROACH PERIPLANETA-AMERICANA; LIGAND-BINDING; PHARMACOLOGICAL CHARACTERIZATION; MOLECULAR-PROPERTIES; MESSENGER-RNAS; BRAIN; MELANOGASTER; SUBTYPES; ANTIBODIES; HYDROLYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
A.D. Blake et al., "DROSOPHILA NERVOUS-SYSTEM MUSCARINIC ACETYLCHOLINE-RECEPTOR - TRANSIENT FUNCTIONAL EXPRESSION AND LOCALIZATION BY IMMUNOCYTOCHEMISTRY", Molecular pharmacology, 44(4), 1993, pp. 716-724

Abstract

The pharmacological properties of a cloned Drosophila muscarinic acetylcholine receptor (mAChR) were investigated using two independent transient expression systems. The binding characteristics of the expressed receptor were determined using transfected COS-7 cells, whereas the mAChR functional properties were analyzed using nuclearly injected Xenopus oocytes. Competition displacement studies with transfected COS-7 cell membranes showed that N-[H-3]methylscopolamine binding was displaced most effectively by atropine, followed by 4-diphenylacetoxy-N-methylpiperidine methiodide, pirenzepine, and AFDX-116. This same order ofeffectiveness (4-diphenylacetoxy-N-methylpiperidine methiodide > pirenzepine > AFDX-116) was observed in oocytes expressing Dm1 when carbamylcholine-induced currents were inhibited by the same antagonists. Thus, the expressed Drosophila mAChR (Dml) exhibits a pharmacology that broadly resembles that of the vertebrate M1 and M3 mAChR subtypes. To determine the anatomical localization of the Drosophila mAChR, polyclonal antiserum was raised against a peptide corresponding to the predicted carboxyl-terminal domain of the receptor. Immunocytochemistry on fly sections demonstrated that the mAChR gene product was found in the nervous system and was not seen in skeletal muscle. The most intense staining was localized to the glomeruli of the antennal lobes, an area of the insect brain where first-order synaptic processing of olfactory information occurs.

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Documento generato il 27/11/20 alle ore 07:04:41