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Titolo:
CHARACTERIZATION OF NEURAMINIDASES PRODUCED BY VARIOUS SEROTYPES OF PASTEURELLA-HAEMOLYTICA
Autore:
STRAUS DC; JOLLEY WL; PURDY CW;
Indirizzi:
TEXAS TECH UNIV,HLTH SCI CTR,DEPT MICROBIOL & IMMUNOL LUBBOCK TX 79430 USDA ARS,CONSERVAT & PROD RES LAB BUSHLAND TX 79012
Titolo Testata:
Infection and immunity
fascicolo: 11, volume: 61, anno: 1993,
pagine: 4669 - 4674
SICI:
0019-9567(1993)61:11<4669:CONPBV>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROUP-B STREPTOCOCCI; EXTRACELLULAR NEURAMINIDASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
D.C. Straus et al., "CHARACTERIZATION OF NEURAMINIDASES PRODUCED BY VARIOUS SEROTYPES OF PASTEURELLA-HAEMOLYTICA", Infection and immunity, 61(11), 1993, pp. 4669-4674

Abstract

Neuraminidases produced by 16 strains of Pasteurella haemolytica (serotypes 1 to 16) were characterized by molecular weight, antigenic identity, and substrate specificity. After growth in a chemically defined medium, stage I (lyophilized) culture supernatants were assayed for activity with N-acetylneuramin lactose, human alpha-1-acid glycoprotein,fetuin, colominic acid, and bovine submaxillary mucin. Neuraminidase produced by P. haemolytica serotype A1 (Ph A1) was purified by a combination of salt fractionation, ion-exchange chromatography on DEAE-Sephacel, and gel filtration on Sephadex G-200. Purified Ph A1 neuraminidase was used to immunize rabbits, and the resultant antiserum reduced the activity of Ph A1 neuraminidase by 46%. This antiserum also reducedthe activity of neuraminidase produced by the other serotypes by between 15 and 66%. Molecular weight estimates of the neuraminidases produced by the various serotypes were obtained by gel filtration chromatography on Sephadex G-200. Fifteen of the 16 serotypes examined produceda neuraminidase with a molecular weight of approximately 150,000 to 200,000. One serotype (serotype 11) produced no material with neuraminidase activity. In addition, all 15 high-molecular-weight neuraminidases showed similar substrate specificities. That is, they were all most active against N-acetylneuramin lactose and least active against bovine submaxillary mucin. On the basis of these results, it appears that the high-molecular-weight neuraminidases produced by the different P. haemolytica serotypes are quite similar.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/10/20 alle ore 12:33:49