Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
(CO2)-C-14 PULSE-CHASE LABELING IN CLUSIA-MINOR L
Autore:
OLIVARES E; FAIST K; KLUGE M; LUTTGE U;
Indirizzi:
TH DARMSTADT,INST BOT,SCHNITTSPAHN STR 3-5 D-64287 DARMSTADT GERMANY TH DARMSTADT,INST BOT,SCHNITTSPAHN STR 3-5 D-64287 DARMSTADT GERMANY IVIC,CTR ECOL CARACAS 1020A VENEZUELA
Titolo Testata:
Journal of Experimental Botany
fascicolo: 267, volume: 44, anno: 1993,
pagine: 1527 - 1533
SICI:
0022-0957(1993)44:267<1527:(PLICL>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
CRASSULACEAN ACID METABOLISM; CAM-TREE CLUSIA; WATER-STRESS; ECOPHYSIOLOGICAL COMPORTMENT; GAS-EXCHANGE; C3-CAM TREE; LIGHT; PLANT; ACCUMULATION; FIXATION;
Keywords:
CLUSIA-MINOR; LABELING; PULSE CHASE; (CO2)-C-14;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
E. Olivares et al., "(CO2)-C-14 PULSE-CHASE LABELING IN CLUSIA-MINOR L", Journal of Experimental Botany, 44(267), 1993, pp. 1527-1533

Abstract

Conditions and maintenance of growth were chosen so that plants of Clusia minor L. were obtained which showed the C3- and CAM- modes of CO2-exchange, respectively. C. minor is known to accumulate considerable amounts of citric acid in addition to malic acid during the dark-phaseof CAM. (CO2)-C-14-pulse-chase experiments were performed with these plants. Patterns of labelling during the pulse and redistribution of label during the chase in the C3-mode were as expected for C3-photosynthesis. Pulse-labelling in the CAM-mode during the last hour of the light period, during the first part of the dark period and during the last hour of the dark period always led to an almost exclusive incorporation of label into malate. Redistribution of label from malate after the pulse at the end of the dark period during the chase in the subsequent light period followed the pattern expected for light-dependent reassimilation of CO2 remobilized from malate in CAM during the light period. During the chases in the dark period, label was transferred from C-14-malate to citrate. This suggests that during accumulation of citric acid in the dark period of CAM in C. minor, citrate is synthesized in the mitochondria from malate or oxaloacetate after formation of malate via phosphoenolpyruvate carboxylase. The experiment also showed that no labelled compounds are exported from leaves in the CAM-mode during the dark period. In plants of the C3-mode the roots proved to be strong sinks.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 11:38:53