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Titolo:
DIFFERENTIAL DISTRIBUTION OF VESICULAR CARRIERS DURING DIFFERENTIATION AND SYNAPSE FORMATION
Autore:
BURSZTAJN S; JONG YJ; BERMAN SA;
Indirizzi:
HARVARD UNIV,MCLEAN HOSP,SCH MED,MAILMAN RES CTR BELMONT MA 02178 HARVARD UNIV,SCH MED,DEPT NEUROL,DIV GERONTOL BOSTON MA 02115 WASHINGTON UNIV,DEPT NEUROBIOL ST LOUIS MO 63110 HARVARD UNIV,SCH MED,DEPT PSYCHIAT,PROGRAM NEUROSCI BELMONT MA 02178
Titolo Testata:
Journal of cellular biochemistry
fascicolo: 3, volume: 53, anno: 1993,
pagine: 251 - 264
SICI:
0730-2312(1993)53:3<251:DDOVCD>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLATHRIN-COATED VESICLES; RECEPTOR-MEDIATED ENDOCYTOSIS; ACETYLCHOLINE-RECEPTORS; MONOCLONAL-ANTIBODIES; PROTEIN-TRANSPORT; CHICK MYOTUBES; MESSENGER-RNA; RAT MYOTUBES; GOLGI STACK; MEMBRANE;
Keywords:
COATED VESICLES; MONOCLONAL ANTIBODIES SYNAPSE FORMATION; NERVE-MUSCLE CULTURES; IMMUNOFLUORESCENT LOCALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
61
Recensione:
Indirizzi per estratti:
Citazione:
S. Bursztajn et al., "DIFFERENTIAL DISTRIBUTION OF VESICULAR CARRIERS DURING DIFFERENTIATION AND SYNAPSE FORMATION", Journal of cellular biochemistry, 53(3), 1993, pp. 251-264

Abstract

Coated and noncoated vesicles participate in cellular protein transport. Both acetylcholine receptors (AChR) and acetylcholinesterase (AChE) are transported via coated vesicles, some of which accumulate beneath the neuromuscular synapse where AChRs cluster. To investigate the mechanisms by which these proteins are transported during postsynaptic remodeling, we purified coated vesicles from the bovine brain via column chromatography (Sephacryl S-1000) and raised monoclonal antibodies to epitopes of the vesicular membranes enriched in AChE. We assayed forAChE (coated vesicle enriched), hexosaminidase (lysosomal contaminants), NADH cytochrome C reductase (mitochondrial containing), and protein and demonstrated electron microscopically using negative staining that the vesicular fraction contained 95% pure coated vesicles. We then injected coated vesicle fractions and the fractions from which the coat was removed intraperitoneally into mice and obtained three monoclonal antibodies: C-33, C-172, and F-22. On immunoblots of purified vesicles and cultured skeletal muscle, mAb C-33 stained a 180 Kd band and mAb C-172 stained a 100 kd band. MAb F-22 stained 50 kd and 55 kd bands and was not characterized further. Immunofluorescent microscopy with C-33 and C-172 revealed punctate fluorescence whose distribution depends upon the stage of myotube development. Four days after plating, myotubes showed punctate fluorescence throughout the myotube, whereas those stained 8 days after plating showed a punctate perinuclear distribution. Myotubes innervated by ciliary neurons show punctate fluorescencelimited to the nuclear periphery and most concentrated around nuclei which line up beneath neuronal processes. This differential vesicular distribution, observed during myotube differentiation and innervation,suggests that these vesicles participate in vesicular membrane traffic. (C) 1993 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 22:13:23