Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DEPRESSION OF PRIMARY AFFERENT-EVOKED RESPONSES BY GR71251 IN THE ISOLATED SPINAL-CORD OF THE NEONATAL RAT
Autore:
GUO JZ; YOSHIOKA K; YANAGISAWA M; HOSOKI R; HAGAN RM; OTSUKA M;
Indirizzi:
TOKYO MED & DENT UNIV,FAC MED,DEPT PHARMACOL TOKYO 113 JAPAN GLAXO GRP RES LTD,DEPT NEUROPHARMACOL WARE SG12 0DP HERTS ENGLAND
Titolo Testata:
British Journal of Pharmacology
fascicolo: 3, volume: 110, anno: 1993,
pagine: 1142 - 1148
SICI:
0007-1188(1993)110:3<1142:DOPARB>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
GASTRIN-RELEASING PEPTIDE; SUBSTANCE-P ANTAGONISTS; NEWBORN RAT; TACHYKININ ANTAGONIST; SENSORY NEURONS; NERVOUS-SYSTEM; DORSAL HORN; BOMBESIN; RECEPTORS; POTENT;
Keywords:
TACHYKININ RECEPTOR; TACHYKININ ANTAGONIST; GR71251; SUBSTANCE-P; BOMBESIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
J.Z. Guo et al., "DEPRESSION OF PRIMARY AFFERENT-EVOKED RESPONSES BY GR71251 IN THE ISOLATED SPINAL-CORD OF THE NEONATAL RAT", British Journal of Pharmacology, 110(3), 1993, pp. 1142-1148

Abstract

1 The pharmacological profile of GR71251, a new tachykinin receptor antagonist, and its effect on the responses evoked by stimulation of primary afferent fibres were studied in isolated spinal cord preparations of neonatal rats. Potential changes were recorded extracellularly from a lumbar ventral root 2 Bath-application of substance P (SP), neurokinin A (NKA) and neurokinin B (NKB) at 0.01-3 muM to the spinal cord induced depolarization of the ventral root in normal artificial cerebrospinal fluid (CSF). The NK1 agonist, acetyl-Arg6-septide, and the NK3agonist, senktide, at 0.01-3 muM, also had potent depolarizing actions whereas two NK2 agonists, beta-Ala8NKA4-10 and Nle10NKA4-10, showed little depolarizing effects at 1 muM. 3 GR71251 (0.3-3 muM) caused a rightward shift of the concentration-response curves for SP, acetyl-Arg6-septide and NKA with pA2 values of 6.14, 6.75 or 6.70, respectively. The effects of GR71251 were readily reversible within 15-30 min afterits removal. By contrast, GR71251 (1-5 muM) had little effect on the depolarizing responses to NKB and senktide. 4 GR71251 (1-3 muM) did not depress the depolarizing responses to bombesin, neuromedin B and gastrin-releasing peptide in normal artificial CSF. 5 Application of capsaicin to the spinal cord induced a depolarizing response, which was partially depressed by GR71251 (3-10 muM). 6 In the isolated spinal cordpreparation, intense electrical stimulation of a dorsal root evoked aslow depolarizing response of the contralateral ventral root of the same segment. A similar slow ventral root depolarization was evoked by electrical stimulation of the ipsilateral saphenous nerve in an isolated spinal cord-saphenous nerve preparation. GR712 51 (0.3-10 muM) dose-dependently depressed these slow ventral root potentials. 7 In the spinal cord-peripheral nerve preparation, conditioning stimulation of the saphenous nerve evoked an inhibition of the muscle nerve-evoked monosynaptic reflex lasting about 20 s. The late part of the inhibition was markedly depressed by GR7125 (1-3 muM). 8 The present results indicate that GR71251 is a potent and specific antagonist for tachykinin receptors in the spinal cord. The present study further provides evidencefor the involvement of SP and NKA in the slow ventral root depolarization and the prolonged inhibition of monosynaptic reflex that are evoked by primary afferent stimulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 23:52:38