Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ROLE OF VOLTAGE-GATED CA2-AMP IN THE PRESENCE OR ABSENCE OF NGF( CHANNELS AND INTRACELLULAR CA2+ IN RAT SYMPATHETIC NEURON SURVIVAL AND FUNCTION PROMOTED BY HIGH K+ AND CYCLIC)
Autore:
MURRELL RD; TOLKOVSKY AM;
Indirizzi:
UNIV OXFORD,DEPT HUMAN ANAT,S PARKS RD OXFORD OX1 3QX ENGLAND UNIV OXFORD,DEPT HUMAN ANAT,S PARKS RD OXFORD OX1 3QX ENGLAND
Titolo Testata:
European journal of neuroscience
fascicolo: 10, volume: 5, anno: 1993,
pagine: 1261 - 1272
SICI:
0953-816X(1993)5:10<1261:ROVCIT>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; CALCIUM CHANNELS; NEURITE OUTGROWTH; CONE MOTILITY; CHRONIC DEPOLARIZATION; CHROMAFFIN CELLS; SENSORY NEURONS; CA-2+ CURRENT; OKADAIC ACID; PROTEIN;
Keywords:
NEURONAL SURVIVAL; CA2+ INFLUX; NEUROTROPHIC FACTORS; CELL DEATH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
R.D. Murrell e A.M. Tolkovsky, "ROLE OF VOLTAGE-GATED CA2-AMP IN THE PRESENCE OR ABSENCE OF NGF( CHANNELS AND INTRACELLULAR CA2+ IN RAT SYMPATHETIC NEURON SURVIVAL AND FUNCTION PROMOTED BY HIGH K+ AND CYCLIC)", European journal of neuroscience, 5(10), 1993, pp. 1261-1272

Abstract

We have examined how NGF-dependent rat sympathetic neurons maintain Ca2+ homeostasis when challenged with high K+ or 8-(4-chlorophenylthio)cyclic AMP (CPTcAMP), two survival factors. In the presence of NGF, high K+ (55 mM) caused a stable, 65% reduction in the density of cell soma voltage-sensitive Ca2+ channels within 2 days. Although resting [Ca2+]i was elevated by 1.6-fold, this was 50% less than the rise in [Ca2]i measured before down-regulation occurred, suggesting that down-regulation may help prevent the toxic effects of persistently elevated [Ca2+]i. Inhibition of protein synthesis by cycloheximide blocked recovery from down-regulation. Moreover, treatment with cycloheximide or actinomycin-D caused a 2-fold rise in the peak Ca2+ current, suggesting that voltage-sensitive Ca2+ channel activity may be tonically attenuated during normal growth. In the absence of NGF, neurons survived for several days in high K+ medium with no significant rise in resting (Ca2+]i, although neurites did not grow. Neither Ca2+ channel density nor resting [Ca2+]i were altered in neurons surviving with CPTcAMP. Moreover, CPTcAMP lowered the dependence on extracellular Ca2+. However, the dihydropyridine antagonist nitrendipine blocked both high K+- and CPTcAMP-dependent survival although it had no effect in the presence of NGF. Thus, in the absence of NGF, sympathetic neurons do not require elevation of [Ca2+]i above resting levels to survive with either high K+ or CPTcAMP, but dihydropyridine-sensitive Ca2+ channel activity may beessential for their survival promoting actions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:14:20