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Titolo:
DETECTION OF ALPRAZOLAM IN HAIR BY NEGATIVE-ION CHEMICAL-IONIZATION MASS-SPECTROMETRY
Autore:
HOLD KM; CROUCH DJ; WILKINS DG; ROLLINS DE; MAES RA;
Indirizzi:
UNIV UTAH,DEPT PHARMACOL & TOXICOL,CTR HUMAN TOXICOL,490 BIOMED POLYMERS BLDG SALT LAKE CITY UT 84112 UNIV UTRECHT,DEPT ANAL & TOXICOL,UTRECHT INST PHARMACEUT SCI NL-3584 CA UTRECHT NETHERLANDS
Titolo Testata:
Forensic science international
fascicolo: 1-3, volume: 84, anno: 1997,
pagine: 201 - 209
SICI:
0379-0738(1997)84:1-3<201:DOAIHB>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
ABBOTT ADX; CONFIRMATION; METABOLITES; DRUGS; BENZODIAZEPINES; TRIAGE(TM); EXTRACTION; TRIAZOLAM; GC/MS;
Keywords:
ALPRAZOLAM; BENZODIAZEPINES; HAIR; GC/MS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
K.M. Hold et al., "DETECTION OF ALPRAZOLAM IN HAIR BY NEGATIVE-ION CHEMICAL-IONIZATION MASS-SPECTROMETRY", Forensic science international, 84(1-3), 1997, pp. 201-209

Abstract

A sensitive and specific method for the quantitative determination ofalprazolam (AL) in hair has been developed. After the addition of deuterium labeled triazolam as an internal standard, hair samples (20 mg)were digested with 1 N NaOH at 40 degrees C overnight. Calibrators containing known concentrations of AL dried onto drug-free hair were also prepared and digested. After digestion, the solution was cooled, adjusted to pH 9 with 6 N HCl and 1 mt of saturated sodium berate buffer was added. The digested solutions were extracted with toluene:methylene chloride (7:3) and the organic phase was evaporated to dryness. Extract residues were treated with BSTFA and 1% TMCS and analyzed on a Finnggan-MAT(TM) mass spectrometer in the negative-ion chemical ionization mode with methane as the reagent gas. Chromatographic separation wasachieved on a Restek-200(TM) capillary column using hydrogen as the carrier gas. The assay was capable of detecting 25 pg/mg of AL and was linear to 250 pg/mg. Intra-assay precision was 11.1% at 25 pg/mg and 5.4% at 150 pg/mg. Inter-assay precision was 11.2% at 25 pg/mg and 5.3%at 150 pg/mg. This method has been used to study the hair incorporation of AL into Long-Evans rats who received 5.0 mg/kg or 7.5 mg/kg i.p.twice a day for 5 days. Preliminary results indicate that the AL concentration in the pigmented and non-pigmented hair on day 14 ranged from 60 to 100 pg/mg. (C) 1997 Elsevier Science Ireland Ltd.

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Documento generato il 24/11/20 alle ore 14:36:30