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Titolo:
REPRESSION OF HISTONE GENE-TRANSCRIPTION IN QUIESCENT 3T6 FIBROBLASTS
Autore:
ZAHRADKA P; ELLIOT T; HOVLAND K; LARSON DE; SAWARD L;
Indirizzi:
UNIV MANITOBA,ST BONIFACE GEN HOSP,RES CTR,DEPT PHYSIOL,DIV CARDIOVASC SCI,351 TACHE AVE MANITOBA R2H 2A6 MB CANADA UNIV GUELPH,DEPT CHEM & BIOCHEM GUELPH N1G 2W1 ONTARIO CANADA UNIV GUELPH,DEPT MOLEC BIOL & GENET GUELPH N1G 2W1 ONTARIO CANADA
Titolo Testata:
European journal of biochemistry
fascicolo: 2, volume: 217, anno: 1993,
pagine: 683 - 690
SICI:
0014-2956(1993)217:2<683:ROHGIQ>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN H4-HISTONE GENE; CELL-CYCLE REGULATION; FACTOR HINF-D; PROTEIN-DNA INTERACTIONS; MESSENGER-RNA LEVELS; INVITRO TRANSCRIPTION; EXPRESSION; DIFFERENTIATION; ELEMENT; UPSTREAM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
P. Zahradka et al., "REPRESSION OF HISTONE GENE-TRANSCRIPTION IN QUIESCENT 3T6 FIBROBLASTS", European journal of biochemistry, 217(2), 1993, pp. 683-690

Abstract

Maintaining murine 3T6 fibroblasts in serum-depleted medium for a period of three days results in a resting cell population that does not synthesize DNA. Histone mRNA levels, closely tied to the cell-proliferation rate, are low due to a reduced rate of synthesis. A comparison ofhistone gene transcription in vitro by nuclear extracts of quiescent or proliferative 3T6 cells showed that a 200-bp segment of the promoter was responsible for repressing gene activity when cells were in a G0state. In the absence of the distal promoter region (- 200 to - 400),gene transcription remained high in quiescent cells, indicating the proximal promoter region (+ 1 to - 200) was responsible for basal gene activity. Alterations in protein binding to the distal promoter regioncorrelated with histone H4 gene activity, suggesting that repression of histone gene transcription is linked to the attachment of a specific nuclear protein. During G1, the histone H4 gene was efficiently transcribed in vitro, but an inability to process the histone pre-mRNA limited the cellular content of mature histone mRNA. This distinction between transcriptional (in G0) and post-transcriptional (in G1) mechanisms for modulating histone mRNA levels suggests that gene-regulatory factors are specifically activated in quiescent cells to reduce expression of non-essential genes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 19:21:27