Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
INDUCTION OF CYTOLYTIC AND ANTIBODY-RESPONSES USING PLASMODIUM-FALCIPARUM REPEATLESS CIRCUMSPOROZOITE PROTEIN ENCAPSULATED IN LIPOSOMES
Autore:
WHITE K; KRZYCH U; GORDON DM; PORTER TG; RICHARDS RL; ALVING CR; DEAL CD; HOLLINGDALE M; SILVERMAN C; SYLVESTER DR; BALLOU WR; GROSS M;
Indirizzi:
WRAMC,WRAIR,DEPT IMMUNOL WASHINGTON DC 20307 WRAMC,WRAIR,DEPT IMMUNOL WASHINGTON DC 20307 WALTER REED ARMY INST RES,DEPT MEMBRANE BIOCHEM WASHINGTON DC 20307 SMITHKLINE BEECHAM PHARMACEUT,DEPT MACROMOLEC SCI KING OF PRUSSIA PA 19406 BIOMED RES INST ROCKVILLE MD 20852 SMITHKLINE BEECHAM PHARMACEUT,DEPT PROT BIOCHEM KING OF PRUSSIA PA 19406 WALTER REED ARMY INST RES,DEPT BACTERIAL DIS WASHINGTON DC 20307
Titolo Testata:
Vaccine
fascicolo: 13, volume: 11, anno: 1993,
pagine: 1341 - 1346
SICI:
0264-410X(1993)11:13<1341:IOCAAU>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
TOXIC T-CELLS; CYTOTOXIC LYMPHOCYTES-T; SPOROZOITE VACCINE; SYNTHETIC PEPTIDE; MALARIA VACCINE; ANTIGEN; IMMUNOGENICITY; SAFETY; PHAGOCYTOSIS; STIMULATION;
Keywords:
MALARIA; REPEATLESS CIRCUMSPOROZOITE PROTEIN; CYTOLYTIC-T CELLS; ANTIBODY; LIPOSOMES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
K. White et al., "INDUCTION OF CYTOLYTIC AND ANTIBODY-RESPONSES USING PLASMODIUM-FALCIPARUM REPEATLESS CIRCUMSPOROZOITE PROTEIN ENCAPSULATED IN LIPOSOMES", Vaccine, 11(13), 1993, pp. 1341-1346

Abstract

Plasmodium circumsporozoite (CS) protein-induced antibody and T-cell responses are considered to be important in protective immunity. Sincethe key repeat determinant of the CS protein may actually restrict the recognition of other potential T- and B-cell sites, a modified Plasmodium falciparum CS protein lacking the central repeat region, RLF, was expressed in Escherichia coli. On purification, RLF was encapsulatedinto liposomes [L(RLF)] and used for the in vivo induction of cytolytic T lymphocytes (CTL) and antibodies. Immunization of B10.Br (H-2k) mice with L(RLF), but not with RLF, induced CD8+ CTL specific for the P. falciparum CS protein CTL epitope, amino acid residues 368-390. Anti-L(RLF) serum reacted with antigens on intact sporozoites and inhibited sporozoite invasion of hepatoma cells. Antibody specificity studies in New Zealand White rabbits revealed new B-cell sites localized in amino acid residues 84-94, 91-99, 97-106 and 367-375. Although the mechanisms by which liposomes enhance cellular and humoral immune responsesremain unknown, liposome-formulated vaccines have been well toleratedin humans; hence, their use in vaccines, when efficacy depends on antibody and CTL responses, may be broadly applicable.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:35:58