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Titolo:
I-125 TYR(0)-HCRH LABELING CHARACTERISTICS OF CORTICOTROPIN-RELEASINGHORMONE RECEPTORS - DIFFERENCES BETWEEN NORMAL AND ADENOMATOUS CORTICOTROPHS
Autore:
ABS R; SMETS G; VAUQUELIN G; VERHELST J; MAHLER C; VERLOOY J; STEVENAERT A; WOUTERS L; BORGERS M; BECKERS A;
Indirizzi:
UNIV ANTWERP,DEPT ENDOCRINOL,UNIV PLEIN 1 B-2610 WILRIJK BELGIUM JANSSEN RES FDN,DEPT MORPHOL B-2340 BEERSE BELGIUM VUB,INST MOL BIOL,DEPT PROT CHEM RHODE ST GENESE BELGIUM UNIV ANTWERP,DEPT NEUROSURG B-2020 ANTWERP BELGIUM UNIV LIEGE,DEPT NEUROSURG LIEGE BELGIUM UNIV LIEGE,DEPT ENDOCRINOL LIEGE BELGIUM MIDDELHEIM HOSP,DEPT ENDOCRINOL ANTWERP BELGIUM
Titolo Testata:
Neurochemistry international
fascicolo: 3, volume: 30, anno: 1997,
pagine: 291 - 297
SICI:
0197-0186(1997)30:3<291:ITLCOC>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR CRF RECEPTORS; RAT PITUITARY-GLAND; AUTORADIOGRAPHIC LOCALIZATION; CUSHINGS-SYNDROME; ADRENOCORTICOTROPIN; BINDING; ADRENALECTOMY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
R. Abs et al., "I-125 TYR(0)-HCRH LABELING CHARACTERISTICS OF CORTICOTROPIN-RELEASINGHORMONE RECEPTORS - DIFFERENCES BETWEEN NORMAL AND ADENOMATOUS CORTICOTROPHS", Neurochemistry international, 30(3), 1997, pp. 291-297

Abstract

The presence of corticotropin-releasing hormone (CRH) receptors has been previously demonstrated in corticotrophs From normal pituitaries using a method combining immunocytochemistry and liquid emulsion autoradiography. The aim of this study was to compare the characteristics ofthe I-125-Tyr(0)-hCRH binding in corticotrophs from normal pituitaries (three obtained at autopsy and one obtained at surgery) with corticotrophs from pituitary adenomas (six corticotroph adenomas responsible for Cushing's disease and two silent corticotroph adenomas secreting abiologically inactive ACTH molecule). In normal corticotrophs, the larger part of the I-125-Tyr(0)-hCRH binding was localised in patchy conglomerates at the centre of the cell and, to a much lesser degree, in a diffuse pattern at the cell periphery. In adenomatous corticotrophs,CRH receptor expression is disturbed both quantitatively and qualitatively. Except for a minority of cells in one adenoma, all adenomatous corticotrophs showed only peripherally bound I-125-Tyr(0)hCRH and no centrally localised binding. Furthermore, adenomatous corticotrophs revealed a statistically significant lower signal intensity when comparedto normal corticotrophs and a strongly negative correlation was foundbetween the labelling area in adenomatous corticotrophs and both the basal and CRH-stimulated plasma ACTH levels. These findings suggest defective processing of CRH receptors and could be relevant to the sustained ACTH secretion by adenomatous corticotrophs in Cushing's disease and, more generally, provide an explanation to its pathology. Thr silent corticotrophs secreting a biologically inactive ACTH molecule were characterised by a very faint signal intensity, although present on almost every cell. (C) 1997 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:52:57