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Titolo:
SUBCLONING OF HIV-2 NEF GENES IN ESCHERICHIA-COLI AND IMMUNOLOGICAL REACTIVITY OF EXPRESSED FUSION PROTEINS
Autore:
ULRICH R; SIAKKOU H; MAYER J; KIENZLE N; MULLERLANTZSCH N; KRUGER DH;
Indirizzi:
HUMBOLDT UNIV BERLIN,MED FAK CHARITE,INST MED VIROL,SCHUMANNSTR 20-21O-1005 BERLIN GERMANY UNIV SAARLAND,INST MED MIKROBIOL & HYG,VIROL ABT W-6600 SAARBRUCKEN GERMANY
Titolo Testata:
Virus genes
fascicolo: 3, volume: 7, anno: 1993,
pagine: 229 - 240
SICI:
0920-8569(1993)7:3<229:SOHNGI>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; MUTATIONAL ANALYSIS; NUCLEOTIDE-SEQUENCE; ANTIBODY-RESPONSE; NEGATIVE FACTOR; PROVIRAL CLONE; CONSTRUCTION; VECTORS; SEROCONVERSION; PRODUCTS;
Keywords:
NEF; HIV-2; EXPRESSION IN ESCHERICHIA-COLI; BETA-GALACTOSIDASE FUSION PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
R. Ulrich et al., "SUBCLONING OF HIV-2 NEF GENES IN ESCHERICHIA-COLI AND IMMUNOLOGICAL REACTIVITY OF EXPRESSED FUSION PROTEINS", Virus genes, 7(3), 1993, pp. 229-240

Abstract

The nef gene located in the 3' region of the HIV-2 genome encodes an N-terminally myristylated protein of 27-35 kD, likely to be involved in the regulation of viral transcription. The nef genes of HIV-2 isolates GH-1, ROD, ST, BEN, and D194.17 were inserted into E. coli pEX vectors and expression of Nef beta-galactosidase fusion proteins was detected in stained gels. All fusion proteins specifically reacted with a rabbit serum raised against bacterially expressed Nef from HIV-2D194.17. Sera from monkeys inoculated with HIV-2BEN or SIV(MAC)251 recognizedthe Nef proteins of only certain HIV-2 isolates. No cross-reactivity of these sera with HIV-1 Nef and of a rabbit anti-HIV-1-Nef serum withthe described HIV-2 Nef fusion proteins was observed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 17:39:12