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Titolo:
A FUNCTIONAL GTP-BINDING MOTIF IS NECESSARY FOR ANTIVIRAL ACTIVITY OFMX PROTEINS
Autore:
PITOSSI F; BLANK A; SCHRODER A; SCHWARZ A; HUSSI P; SCHWEMMLE M; PAVLOVIC J; STAEHELI P;
Indirizzi:
UNIV FREIBURG,DEPT VIROL D-79008 FREIBURG GERMANY UNIV FREIBURG,DEPT VIROL D-79008 FREIBURG GERMANY UNIV ZURICH,INST VIROL CH-8028 ZURICH SWITZERLAND
Titolo Testata:
Journal of virology
fascicolo: 11, volume: 67, anno: 1993,
pagine: 6726 - 6732
SICI:
0022-538X(1993)67:11<6726:AFGMIN>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
GDP DISSOCIATION INHIBITOR; MESSENGER-RNA SYNTHESIS; INFLUENZA-VIRUS; RAS ONCOGENE; DYNAMIN; DOMAIN; YEAST; EXPRESSION; RESISTANCE; PRODUCT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
F. Pitossi et al., "A FUNCTIONAL GTP-BINDING MOTIF IS NECESSARY FOR ANTIVIRAL ACTIVITY OFMX PROTEINS", Journal of virology, 67(11), 1993, pp. 6726-6732

Abstract

Mx proteins are interferon-induced GTPases that inhibit the multiplication of certain negative-stranded RNA viruses. However, it has been unclear whether GTPase activity is necessary for antiviral function. Here, we have introduced mutations into the tripartite GTP-binding consensus elements of the human MxA and mouse Mx1 proteins. The invariant lysine residue of the first consensus motif, which interacts with the beta- and gamma-phosphates of bound GTP in other GTPases, was deleted or replaced by methionine or alanine. These Mx mutants and appropriate controls were then tested for antiviral activity, GTP-binding capacity, and GTPase activity. We found a direct correlation between the GTP-binding capacities and GTP hydrolysis activities of the purified Mx mutants in vitro and their antiviral activities in transfected 3T3 cells,demonstrating that a functional GTP-binding motif is necessary for virus inhibition. Our results, thus, firmly establish antiviral activityas a novel function of a GTPase, emphasizing the enormous functional diversity of GTPase superfamily members.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:44:24