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Titolo:
DIFFERENTIAL TRANSCRIPTION AND TRANSLATION OF IMMEDIATE-EARLY GENES IN THE GERBIL HIPPOCAMPUS AFTER TRANSIENT GLOBAL-ISCHEMIA
Autore:
KIESSLING M; STUMM G; XIE YX; HERDEGEN T; AGUZZI A; BRAVO R; GASS P;
Indirizzi:
UNIV HEIDELBERG,DEPT NEUROPATHOL,INST NEUROPATHOL,NEUENHEIMER FELD 220 W-6900 HEIDELBERG GERMANY UNIV HEIDELBERG,INST PHYSIOL 2 W-6900 HEIDELBERG GERMANY JANSSEN RES FDN NEUSS GERMANY INST MOLEC PATOL VIENNA AUSTRIA BRISTOL MYERS SQUIBB PHARMACEUT RES INST,DEPT MOLEC BIOL PRINCETON NJ00000
Titolo Testata:
Journal of cerebral blood flow and metabolism
fascicolo: 6, volume: 13, anno: 1993,
pagine: 914 - 924
SICI:
0271-678X(1993)13:6<914:DTATOI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
DELAYED NEURONAL DEATH; C-FOS; PROTEIN-SYNTHESIS; RAT-BRAIN; JUN-B; INSITU HYBRIDIZATION; BINDING AFFINITIES; FOREBRAIN ISCHEMIA; GROWTH-FACTORS; AP-1 SITE;
Keywords:
FOS; JUN; KROX-24; IN-SITU HYBRIDIZATION; IMMUNOHISTOCHEMISTRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
M. Kiessling et al., "DIFFERENTIAL TRANSCRIPTION AND TRANSLATION OF IMMEDIATE-EARLY GENES IN THE GERBIL HIPPOCAMPUS AFTER TRANSIENT GLOBAL-ISCHEMIA", Journal of cerebral blood flow and metabolism, 13(6), 1993, pp. 914-924

Abstract

Excitotoxic activation of glutamate receptors is thought to be a key event for the molecular pathogenesis of postischemic delayed neuronal death of CA-1 neurons in the gerbil hippocampus. Glutamate receptor stimulation also causes induction of transcription factors that belong to the class of immediate early genes. We examined the expression of six different immediate early genes in the gerbil hippocampus after transient global ischemia. Comparative analysis of c-fos and Krox-24 expression was carried out in the same animals at the transcriptional and translational level by in situ hybridization and immunocytochemistry. Postischemic synthesis of four additional immediate early gene (IEG)-encoded proteins (FOS-B, c-JUN, JUN-B, and JUN-D) was investigated by immunocytochemistry at recirculation intervals between 1 and 48 h. After5 min of ischemia, transcription of c-fos and Krox-24 mRNA was induced in all hippocampal subpopulations with peak expression at 1 h after recirculation. In vulnerable CA-1 neurons, increased transcription of c-fos and Krox-24 was not followed by translation into protein. Induction of immediate early gene-encoded proteins was restricted to neuronal populations less vulnerable to brief ischemia and identified neuronsthat are targets of glutamate receptor-mediated neurotoxicity but that are destined to survive. Our data indicate an asynchronous synthesisand persistence of individual IEG-encoded proteins in these neurons. The staggered induction implies that combinatorial changes of transcription factors allow a differential postischemic regulation of target gene expression both spatially and over time.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 13:29:50