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Titolo:
CHARACTERIZATION AND CHROMOSOMAL LOCALIZATION OF THE HUMAN PROTOONCOGENE BMI-1
Autore:
ALKEMA MJ; WIEGANT J; RAAP AK; BERNS A; VANLOHUIZEN M;
Indirizzi:
UNIV AMSTERDAM,NETHERLANDS CANC INST,DIV MOLEC GENET,PLESMANLAAN 121 1066 CX AMSTERDAM NETHERLANDS UNIV AMSTERDAM,NETHERLANDS CANC INST,DIV MOLEC GENET,PLESMANLAAN 121 1066 CX AMSTERDAM NETHERLANDS UNIV AMSTERDAM,NETHERLANDS CANC INST,DEPT BIOCHEM 1066 CX AMSTERDAM NETHERLANDS LEIDEN UNIV,DEPT CYTOCHEM & CYTOMETRY 2333 AL LEIDEN NETHERLANDS
Titolo Testata:
Human molecular genetics
fascicolo: 10, volume: 2, anno: 1993,
pagine: 1597 - 1603
SICI:
0964-6906(1993)2:10<1597:CACLOT>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYC TRANSGENIC MICE; ACUTE LEUKEMIAS; HOMEOBOX GENE; DROSOPHILA-TRITHORAX; EXPRESSION; SEQUENCE; PROTEINS; LYMPHOMAGENESIS; TRANSLOCATIONS; DRIVEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
M.J. Alkema et al., "CHARACTERIZATION AND CHROMOSOMAL LOCALIZATION OF THE HUMAN PROTOONCOGENE BMI-1", Human molecular genetics, 2(10), 1993, pp. 1597-1603

Abstract

The proto-oncogene bmi-1 is frequently activated by Moloney murine leukemia proviral insertions in Emu-myc transgenic mice1,2. Using a mouse bmi-1 cDNA probe a transcript of 3.3 kb was detected on Northern blots of human Burkitts lymphoma cell lines. We have isolated and sequenced cDNA clones from a human erythroleukemia cell line (K562) derived cDNA library, using different mouse bmi-1 cDNA fragments as a probe. Analysis of genomic BMI-1 sequences reveals a gene structure which is very similar to that of the mouse, consisting of at least 10 exons. The human cDNA is 3203 bp in length and shows 86% identity to the mouse nucleotide sequence. The open reading frame encodes a protein of 326 amino acids which shares 98% identity to the amino acid sequence of mousebmi-1 protein. In vitro translation experiments show that human cDNA derived RNA translates into a protein with a mobility of 44 - 46 kD onSDS polyacrylamide gels. Fluorescence in situ hybridization (FISH) onmetaphase chromosome spreads located the human BMI-1 gene to the short arm of chromosome 10 (10p13), a region known to be involved in translocations in various leukemias.

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Documento generato il 28/11/20 alle ore 14:30:43