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Titolo:
QUANTITATIVE-ANALYSIS OF HER-2 NEU (ERBB2) GENE-EXPRESSION USING REVERSE-TRANSCRIPTASE POLYMERASE CHAIN-REACTION/
Autore:
SARKAR FH; VISSCHER DW; CRISSMAN JD;
Indirizzi:
WAYNE STATE UNIV,SCH MED,DEPT PATHOL,540 E CANFIELD DETROIT MI 48201 HARPER GRACE HOSP DETROIT MI 48201
Titolo Testata:
Diagnostic molecular pathology
fascicolo: 3, volume: 2, anno: 1993,
pagine: 210 - 218
SICI:
1052-9551(1993)2:3<210:QOHN(G>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
DUCTAL CARCINOMA INSITU; GROWTH-FACTOR RECEPTOR; HUMAN-BREAST CARCINOMA; MESSENGER-RNA; NEU ONCOGENE; IMMUNOHISTOCHEMICAL EVALUATION; C-ERBB-2 EXPRESSION; CANCER; AMPLIFICATION; PROTEIN;
Keywords:
ERBB2; BREAST CARCINOMA; POLYMERASE CHAIN REACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
F.H. Sarkar et al., "QUANTITATIVE-ANALYSIS OF HER-2 NEU (ERBB2) GENE-EXPRESSION USING REVERSE-TRANSCRIPTASE POLYMERASE CHAIN-REACTION/", Diagnostic molecular pathology, 2(3), 1993, pp. 210-218

Abstract

Inappropriate expression of Her-2/neu (ERBB2) gene has been associated with impaired breast cancer prognosis, suggesting a functional role in tumor progression. Herein we describe a quantitative method for analysis of Her-2/neu gene messenger RNA (mRNA), which employs reverse transcriptase polymerase chain reaction (RT-PCR) on a 10-mum cryostat section. The technique combines modified RNA extraction with complementary DNA (cDNA) synthesis to achieve a high level of sensitivity. Utilizing this PCR-based gene expression assay, we were able to quantitate variable amounts of Her-2/neu mRNA in cell lines with established levels of gene expression and in clinical human breast cancer specimens. Inclinical samples, mRNA levels correlated with intensity of immunoperoxidase staining for corresponding oncoprotein. We conclude that PCR-based mRNA quantitation can be applied to quantitative analysis of Her-2/neu gene expression, and potentially many other genes, in samples of limited size.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 19:39:05