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Titolo:
DETECTION OF HUMAN T-LYMPHOTROPIC VIRUS TYPE-I TAX GENE IN SALIVARY-GLAND EPITHELIUM FROM 2 PATIENTS WITH SJOGRENS-SYNDROME
Autore:
MARIETTE X; AGBALIKA F; DANIEL MT; BISSON M; LAGRANGE P; BROUET JC; MORINET F;
Indirizzi:
HOP ST LOUIS,SERV IMMUNOHEMATOL,1 AVE CLAUDE VELLEFAUX F-75010 PARIS FRANCE HOP BICETRE,RHEUMATOL SERV LE KREMLIN BICETR FRANCE HOP ST LOUIS,IMMUNOL HEMATOL SERV,IMMUNOPATHOL LAB F-75010 PARIS FRANCE HOP ST LOUIS,BACTERIOL VIROL SERV F-75010 PARIS FRANCE HOP ST LOUIS,CENT HEMATOL LAB F-75010 PARIS FRANCE
Titolo Testata:
Arthritis and rheumatism
fascicolo: 10, volume: 36, anno: 1993,
pagine: 1423 - 1428
SICI:
0004-3591(1993)36:10<1423:DOHTVT>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN PAPILLOMA-VIRUS; HTLV-I; CELL-LEUKEMIA; REACTION AMPLIFICATION; INSITU HYBRIDIZATION; ENDOGENOUS SEQUENCE; BIOPSY SPECIMENS; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
X. Mariette et al., "DETECTION OF HUMAN T-LYMPHOTROPIC VIRUS TYPE-I TAX GENE IN SALIVARY-GLAND EPITHELIUM FROM 2 PATIENTS WITH SJOGRENS-SYNDROME", Arthritis and rheumatism, 36(10), 1993, pp. 1423-1428

Abstract

Objective. To investigate whether human T lymphotropic virus type I (HTLV-I) could be involved in the pathogenesis of Sjogren's syndrome (SS). Methods. Labial salivary gland (LSG) biopsy specimens from 9 patients with SS (4 with primary SS and 5 with SS secondary to rheumatoid arthritis) and 9 controls were studied for the presence of the tax geneof HTLV-I using in situ hybridization, and for the presence of tax, gag, pol, and env genes of HTLV-I using the polymerase chain reaction (PCR). Testing for antibodies to HTLV-I and examination of lymphocytes on blood smears were performed to determine whether systemic viral infection was present. Results. Using in situ hybridization and PCR, we detected the tar gene, but not the gag, pol, or env genes, of HTLV-I inLSG sections from 2 of 9 patients with SS and from none of the control subjects. Tax DNA was present mostly in nuclei of epithelial cells, but also in some lymphoid cells. Serum of the 2 affected patients did not contain antibodies to HTLV-I. In 1 patient, examination of blood smears revealed rare convoluted lymphocytes, sometimes with the appearance of ''flower cells,'' as observed in the blood of HTLV-I-infected patients. Conclusion. None of the known endogenous retroviral sequencesis homologous to the tax gene. Thus, we suggest that HTLV-I (or another related retrovirus) can infect salivary epithelium. Transactivationproperties of the tax protein could be implicated in the pathogenesisof SS. Alternatively, viral infection could cause de novo expression of HLA-DR antigens and favor the presentation of antigens by epithelial cells, leading, in some genetically predetermined subjects, to lymphoid infiltration of the gland.

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Documento generato il 27/09/20 alle ore 19:30:33