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Titolo:
MODULATION OF HUMAN T-CELL RESPONSES BY NITRIC-OXIDE AND ITS DERIVATIVE, S-NITROSOGLUTATHIONE
Autore:
MERRYMAN PF; CLANCY RM; HE XY; ABRAMSON SB;
Indirizzi:
HOSP JOINT DIS & MED CTR,DEPT RHEUMATOL,301 E 17TH ST NEW YORK NY 10003 HOSP JOINT DIS & MED CTR,DEPT RHEUMATOL & MOLEC MED NEW YORK NY 10003 NYU,SCH MED,DEPT MED,DIV RHEUMATOL NEW YORK NY 10003
Titolo Testata:
Arthritis and rheumatism
fascicolo: 10, volume: 36, anno: 1993,
pagine: 1414 - 1422
SICI:
0004-3591(1993)36:10<1414:MOHTRB>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-PLATELET AGGREGATION; L-ARGININE; RELAXING FACTOR; RIBONUCLEOTIDE REDUCTASE; MURINE MACROPHAGES; ADENOCARCINOMA CELLS; MOLECULAR-CLONING; HUMAN-NEUTROPHILS; SUPEROXIDE ANION; ENDOTHELIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
P.F. Merryman et al., "MODULATION OF HUMAN T-CELL RESPONSES BY NITRIC-OXIDE AND ITS DERIVATIVE, S-NITROSOGLUTATHIONE", Arthritis and rheumatism, 36(10), 1993, pp. 1414-1422

Abstract

Objective. To examine the effects of nitric oxide (NO) and its more stable derivative, S-nitrosoglutathione (SNO-GSH), on the response of activated T lymphocytes. Methods. The effects of NO and SNO-GSH on DNA synthesis, interleukin-2 (IL-2) production, IL-2 receptor expression, and cGMP accumulation were determined in phytohemagglutinin-activated peripheral blood mononuclear cells (PBMC) and spleen T cells. Results. Nitric oxide (half-life [T1/2] < 15 seconds) did not inhibit T cell proliferation. However, the derivative SNO-GSH (25 muM) (T1/2 >2 hours)inhibited DNA synthesis by a mean +/- SD of 65 +/- 19.6% (P < 0.001) in PBMC and 75 +/- 15% (P < 0.001) in spleen cells. Macrophage depletion of PBMC did not abrogate the inhibition. SNO-GSH had no effect on IL-2 production or IL-2 receptor expression. NO (25 muM) increased the cGMP content of PBMC (0.65 +/- 0.15 pmoles/10(6) cells; P < 0.04), as did SNO-GSH (25 muM) in both PBMC (3.8 +/- 1; P < 0.001) and spleen T cells (5.2 +/- 1.2; P < 0.001). Methylene blue and hemoglobin, which are NO inhibitors, inhibited SNO-GSH-induced cGMP accumulation (P < 0.001). Conclusion. SNO-GSH inhibits T cell DNA synthesis independently of IL-2 production and in association with cGMP accumulation via a NO-dependent mechanism. We suggest that NO and its S-nitrosothiol derivatives may act as endogenous inhibitors of T cell-mediated inflammation.

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Documento generato il 28/09/20 alle ore 19:42:23