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Titolo:
CATECHOLAMINE-DEPENDENT AND VOLUME-DEPENDENT ION FLUXES IN CARP (CYPRINUS-CARPIO) RED-BLOOD-CELLS
Autore:
ORLOV SN; SKRYABIN GA;
Indirizzi:
3850 ST URBAIN PAVILLON MARIE DE LA FERRE MONTREAL H2W 1T8 PQ CANADA MOSCOW MV LOMONOSOV STATE UNIV,SCH BIOL,PHYSICOCHEM BIOMEMBRANES LAB MOSCOW 119899 RUSSIA
Titolo Testata:
Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology
fascicolo: 5, volume: 163, anno: 1993,
pagine: 413 - 420
SICI:
0174-1578(1993)163:5<413:CAVIFI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMOGLOBIN-OXYGEN-AFFINITY; RAINBOW-TROUT; RAT ERYTHROCYTES; SALMO-GAIRDNERI; NA+/H+ EXCHANGE; ADRENERGIC RESPONSES; SKATE ERYTHROCYTES; CATION-TRANSPORT; MEMBRANE; MOVEMENTS;
Keywords:
NA+,K+-PUMP; NA+/H+-EXCHANGE; ANION TRANSPORT; ERYTHROCYTES; CARP, CYPRINUS-CARPIO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
S.N. Orlov e G.A. Skryabin, "CATECHOLAMINE-DEPENDENT AND VOLUME-DEPENDENT ION FLUXES IN CARP (CYPRINUS-CARPIO) RED-BLOOD-CELLS", Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, 163(5), 1993, pp. 413-420

Abstract

In carp erythrocytes, noradrenaline (10(-6) mol . l-1) induces a 30- to 40-fold activation of Na+/H+ exchange (the ethylisopropylamiloride-inhibited component of the Na-22 influx) and a fourfold stimulation ofthe Na+, K+ pump (ouabain-inhibited component of Rb-86 influx). In both cases the effect of noradrenaline is blocked by propranolol but notphentolamine and is imitated by forskolin. An activator of protein kinase C (beta-phorbol 12-myristate, 13-acetate) increases Na+/H+ exchange by 10 times and decreases the Na+, K + pump activity by 20-30 percent. In the presence of ethylisopropylamiloride the increment of the Na, K+ pump activity induced by noradrenaline is reduced by 35-45 percent, indicating the existence of a Na+/H+ exchange-independent mechanism of the Na+, K+ pump regulation by beta-adrenergic catecholamines. Hypertonic shrinkage of carp erythrocytes results in a 40- to 80-fold activation of Na+/H+ exchange, whereas hypotonic swelling induces an increase in the rate of Rb-86+ efflux which is inhibited by furosemide byabout 30-40 percent. The rate of pH0 recovery in response to acidification or alkalinization in rat erythrocytes is approximately 15 times as fast as in carp erythrocytes. Unlike in rat erythrocytes, valinomycin does not cause an alkalinization of incubation medium in carp erythrocytes indicating the absence of conductive pathway in the operation of anion transporter protein. A scheme is suggested which describes the interrelation of Na+/H+ exchange, Na+, K+ pump and a non-identified system providing for K+ efflux in cell swelling, regulation of cell volume and cytoplasmic pH in fish erythrocytes under conditions of deep hypoxia and high activity.

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Documento generato il 27/10/20 alle ore 03:37:43