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Titolo:
BIOCHEMICAL-CHARACTERIZATION OF ISOLATED BRANCHIAL MITOCHONDRIA-RICH CELLS OF OREOCHROMIS-MOSSAMBICUS ACCLIMATED TO FRESH-WATER OR HYPERHALINE SEA-WATER
Autore:
KULTZ D; JURSS K;
Indirizzi:
OREGON STATE UNIV,DEPT ZOOL,CORDLEY HALL 3029 CORVALLIS OR 97331 UNIV ROSTOCK,FACHBEREICH BIOL,WISSENSCH BEREICH TIERPHYSIOL D-18055 ROSTOCK GERMANY
Titolo Testata:
Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology
fascicolo: 5, volume: 163, anno: 1993,
pagine: 406 - 412
SICI:
0174-1578(1993)163:5<406:BOIBMC>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAINBOW-TROUT SALMO; ACID-BASE REGULATION; K+-ATPASE ACTIVITY; CHLORIDE CELLS; FISH GILL; SUCCINIC-DEHYDROGENASE; FUNDULUS-HETEROCLITUS; GAIRDNERI RICHARDSON; ANGUILLA-ANGUILLA; ION-TRANSPORT;
Keywords:
SALINITY; GILL; MITOCHONDRIA-RICH CELLS; TILAPIA, OREOCHROMIS-MOSSAMBICUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
D. Kultz e K. Jurss, "BIOCHEMICAL-CHARACTERIZATION OF ISOLATED BRANCHIAL MITOCHONDRIA-RICH CELLS OF OREOCHROMIS-MOSSAMBICUS ACCLIMATED TO FRESH-WATER OR HYPERHALINE SEA-WATER", Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, 163(5), 1993, pp. 406-412

Abstract

Mitochondria-rich cells have been separated from other epithelial cells of tilapia (Oreochromis mossambicus) gills by density gradient centrifugation on Percoll. During centrifugation two main bands of cells formed. The viability of the cells in both bands was high (>90%). In one band, 45-74% of the total cell number was mitochondria-rich cells. The other band contained at least 80% pavement cells, representing the majority of other gill epithelial cell types. A comparison of the activities of four enzymes involved in major metabolic and ion regulatory functions was made between these two different fractions of cells. Furthermore, the separation of gill epithelial cells and determination ofenzymatic activity was carried out in tilapia after the fish were acclimated to fresh water or hyperhaline sea water (60 mg . ml-1 S) to gain an indication of the relative contribution of mitochondria-rich cells and pavement cells to both NaCl excretion and absorption. Regardless of acclimation salinity, the activities of Na+/K+-ATPase, glutamate dehydrogenase and glucose-6-phosphate dehydrogenase were significantlyhigher in mitochondria-rich cells than in pavement cells. However, tilapia acclimated to hyperhaline sea water possessed significantly lower carbonic anhydrase activity in mitochondria-rich cells than in pavement cells. In contrast, no significant difference of carbonic anhydrase activity was observed between the two cell fractions in tilapia acclimated to fresh water.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:26:17