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Titolo:
SACCHAROMYCES-CEREVISIAE - AN ALTERNATIVE SOURCE FOR HUMAN MICROSOMALLIVER-ENZYMES AND ITS USE IN DRUG-INTERACTION STUDIES
Autore:
EUGSTER HP; SENGSTAG C;
Indirizzi:
SWISS FED INST TECHNOL,INST TOXICOL,SCHORENSTR 16 CH-8603 SCHWERZENBACH SWITZERLAND SWISS FED INST TECHNOL,INST TOXICOL,SCHORENSTR 16 CH-8603 SCHWERZENBACH SWITZERLAND
Titolo Testata:
Toxicology
fascicolo: 1-3, volume: 82, anno: 1993,
pagine: 61 - 73
SICI:
0300-483X(1993)82:1-3<61:S-AASF>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPOXIDE HYDROLASE; CARBAMAZEPINE; VALPROMIDE; INVITRO; KETOCONAZOLE; METABOLISM; CAFFEINE; EXPRESSION; INHIBITION; VALPROATE;
Keywords:
CARBAMACEPINE; CYTOCHROME-P450; DRUG INTERACTION; KETOCONAZOLE; MICROSOMAL EPOXIDE HYDROLASE; VALPROMIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
H.P. Eugster e C. Sengstag, "SACCHAROMYCES-CEREVISIAE - AN ALTERNATIVE SOURCE FOR HUMAN MICROSOMALLIVER-ENZYMES AND ITS USE IN DRUG-INTERACTION STUDIES", Toxicology, 82(1-3), 1993, pp. 61-73

Abstract

Heterologous expression of human cDNAs in the yeast Saccharomyces cerevisiae represents an attractive alternative source of human enzymes and allows metabolic studies to be performed without the need of human tissue. Here we report on the functional expression of human microsomal epoxide hydrolase (hmEH) and cytochrome P450 1A1 and 1A2 in yeast. Microsomal fractions of corresponding yeast strains exhibited enzyme specific activities which allowed the characterization of the heterologous enzymes. The use of these microsomes enabled us to study drug interactions on the respective enzymes with pharmacologically relevant drugs such as carbamazepine epoxide, valpromide and ketoconazole.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/21 alle ore 02:37:32