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Titolo:
EXPRESSION OF HUMAN CYTOCHROME-P450 ENZYMES IN YEAST AND BACTERIA ANDRELEVANCE TO STUDIES ON CATALYTIC SPECIFICITY
Autore:
GUENGERICH FP; GILLAM EMJ; OHMORI S; SANDHU P; BRIAN WR; SARI MA; IWASAKI M;
Indirizzi:
VANDERBILT UNIV,MED CTR,SCH MED,DEPT BIOCHEM NASHVILLE TN 37232 VANDERBILT UNIV,MED CTR,SCH MED,CTR MOLEC TOXICOL NASHVILLE TN 37232
Titolo Testata:
Toxicology
fascicolo: 1-3, volume: 82, anno: 1993,
pagine: 21 - 37
SICI:
0300-483X(1993)82:1-3<21:EOHCEI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
OXIDATIVE DRUG-METABOLISM; HUMAN-LIVER-MICROSOMES; SACCHAROMYCES-CEREVISIAE; ESCHERICHIA-COLI; GENETIC-POLYMORPHISM; NIFEDIPINE OXIDASE; MESSENGER-RNA; IDENTIFICATION; FORM; TOLBUTAMIDE;
Keywords:
AFLATOXIN-B1; CYTOCHROME-P450; CYTOCHROME-P450 3A4; ESCHERICHIA-COLI; SACCHAROMYCES-CEREVESIAE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
F.P. Guengerich et al., "EXPRESSION OF HUMAN CYTOCHROME-P450 ENZYMES IN YEAST AND BACTERIA ANDRELEVANCE TO STUDIES ON CATALYTIC SPECIFICITY", Toxicology, 82(1-3), 1993, pp. 21-37

Abstract

Heterologous expression systems can be utilized to great advantage inthe study of cytochrome P450 (P450) and other enzymes involved in thebiotransformation of drugs and other xenobiotics. The list of studiesmade possible with the technology includes discernment of catalytic specificity, elucidation of structure-activity relationships, and various biophysical measurements. There are advantages and disadvantages toeach of the vector systems and choices must be made on the basis of needs. Yeast expression systems were used to establish that different P450 2C enzymes are involved in the hydroxylations of tolbutamide and (S)-mephenytion. P450 3A4 was also expressed in yeast and its very broad catalytic specificity was confirmed. Recently, it has been possible to express P450 3A4 as well as other human and animal P450s in bacteria after slight modification of their 5'-coding sequences.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 06:24:11