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Titolo:
PURIFICATION AND ANTIPATHOGENIC ACTIVITY OF LIPID TRANSFER PROTEINS (LTPS) FROM THE LEAVES OF ARABIDOPSIS AND SPINACH
Autore:
SEGURA A; MORENO M; GARCIAOLMEDO F;
Indirizzi:
UPM,ETS INGN AGRON,BIOQUIM & BIOL MOLEC LAB E-28040 MADRID SPAIN
Titolo Testata:
FEBS letters
fascicolo: 3, volume: 332, anno: 1993,
pagine: 243 - 246
SICI:
0014-5793(1993)332:3<243:PAAAOL>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Keywords:
ARABIDOPSIS; LIPID TRANSFER PROTEIN; PLANT PATHOGEN; SPINACH; THIONIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
A. Segura et al., "PURIFICATION AND ANTIPATHOGENIC ACTIVITY OF LIPID TRANSFER PROTEINS (LTPS) FROM THE LEAVES OF ARABIDOPSIS AND SPINACH", FEBS letters, 332(3), 1993, pp. 243-246

Abstract

Two homogeneous proteins active in vitro against the bacterial pathogen Clavibacter michiganensis subsp. sepedonicus were obtained from a crude cell-wall preparation from the leaves of Columbia wild-type Arabidopsis. The N-terminal amino acid sequences of these proteins allowed their identification as lipid transfer proteins (LTP-a1, LTP-a2); the LTP1-a1 sequence was identical to that deduced from a previously described cDNA (EMBL M80566) and LTP-a2 was quite divergent (44% identical positions). These proteins were not detected in the cytoplasmic fraction by Western-blot analysis. Proteins LTP-s1 and LTP-s2 were similarlyobtained from spinach leaves; LTP-s1 was 91% identical to a previously purified spinach LTP (Swiss Prot P10976), and LTP-s2 was moderately divergent (71% identical positions). About 1/3 of the total LTPs were detected in the cytoplasmic fraction from spinach by Western-blot analysis. Concentrations of these proteins causing 50% inhibition (EC-50) were in the 0.1-1 muM range for the bacterial pathogens C michiganensis and Pseudomonas solanacearum and close to 10 muM for the fungal pathogen Fusarium solani.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 12:15:39