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Titolo:
EVIDENCE FOR A SINGLE NAPHTHYLPHTHALAMIC ACID-BINDING SITE ON THE ZUCCHINI PLASMA-MEMBRANE
Autore:
MUDAY GK; BRUNN SA; HAWORTH P; SUBRAMANIAN M;
Indirizzi:
WAKE FOREST UNIV,DEPT BIOL,BOX 7325 WINSTON SALEM NC 27109 SANDOZ AGRO INC PALO ALTO CA 94304
Titolo Testata:
Plant physiology
fascicolo: 2, volume: 103, anno: 1993,
pagine: 449 - 456
SICI:
0032-0889(1993)103:2<449:EFASNA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
AUXIN TRANSPORT CARRIER; NET IAA UPTAKE; N-1-NAPHTHYLPHTHALAMIC ACID; PHYTOTROPIN-BINDING; CORN COLEOPTILES; CUCURBITA-PEPO; RECEPTOR; PROTEIN; HYPOCOTYLS; VESICLES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
G.K. Muday et al., "EVIDENCE FOR A SINGLE NAPHTHYLPHTHALAMIC ACID-BINDING SITE ON THE ZUCCHINI PLASMA-MEMBRANE", Plant physiology, 103(2), 1993, pp. 449-456

Abstract

The binding of [2,3,4,5,(n)-H-3]N-1-napthylphthalamic acid ([H-3]NPA)to zucchini (Cucurbita pepo L.) plasma membranes was examined in detail using two different filtration assays and the results were rigorously analyzed by saturation curves, double-reciprocal plots, Scatchard plots, Hill plots, and the computer program Ligand (P.J. Munson, D. Rodbard [1980] Anal Biochem 107:220-239). To facilitate these analyses, anew assay that allows rapid and quantitative analysis of [H-3]NPA binding with high reproducibility and ease of manipulation has been developed. These detailed kinetic analyses indicate that only one binding site for [H-3]NPA (K(d) = 16 nm) was associated with the zucchini plasma membrane. Analysis of [H-3]NPA dissociation by several auxin transport inhibitors revealed similar dissociation constants with both plasmaand microsomal membrane. Collectively, these data indicate the presence of only one binding site for NPA associated with the zucchini plasma membrane.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 09:35:37