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Titolo:
IL-12 INDUCES THE PRODUCTION OF IFN-GAMMA BY NEONATAL HUMAN CD4 T-CELLS
Autore:
WU CY; DEMEURE C; KINIWA M; GATELY M; DELESPESSE G;
Indirizzi:
UNIV MONTREAL,NOTRE DAME HOSP,RES CTR,1560 SHERBROOKE ST E MONTREAL H2L 4M1 PQ CANADA UNIV MONTREAL,NOTRE DAME HOSP,RES CTR,1560 SHERBROOKE ST E MONTREAL H2L 4M1 PQ CANADA HOFFMANN LA ROCHE INC,DEPT INFLAMMAT AUTOIMMUNE DIS NUTLEY NJ 07110
Titolo Testata:
The Journal of immunology
fascicolo: 4, volume: 151, anno: 1993,
pagine: 1938 - 1949
SICI:
0022-1767(1993)151:4<1938:IITPOI>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; LYMPHOCYTE MATURATION FACTOR; LYMPHOKINE GENE-EXPRESSION; BLOOD MONONUCLEAR-CELLS; STIMULATORY FACTOR NKSF; HUMAN PERIPHERAL-BLOOD; MESSENGER-RNA; DISTINCT SUBPOPULATIONS; HETERODIMERIC CYTOKINE; MONOCLONAL-ANTIBODY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
C.Y. Wu et al., "IL-12 INDUCES THE PRODUCTION OF IFN-GAMMA BY NEONATAL HUMAN CD4 T-CELLS", The Journal of immunology, 151(4), 1993, pp. 1938-1949

Abstract

A major difference between ''naive'' and ''memory'' or ''effector'' Th cells is the spectrum of cytokines that they are capable of producing. After stimulation naive cells produce only IL-2, whereas memory cells produce several cytokines including IFN-gamma and IL-4. Using umbilical cord blood-derived CD4 T cells as a source of naive T cells, we first report that these cells are capable of producing large amounts ofIFN-gamma when cultured with low concentrations of IL-1 2. The response is time- and dose-dependent, and it is observed at the protein and mRNA levels. IL-1 2 also induces neonatal CD4 T cells to produce lymphotoxin but not IL-2, TNF-alpha, or IL-4. The production of IFN-gamma by IL-1 2-stimulated neonatal T cells is associated with a small but significant T cell activation evidenced by DNA synthesis and by the expression of the activation markers CD25, CD71, and HLA-DR; moreover, it is inhibited by hydrocortisone, cyclosporin A, and transforming growthfactor-beta. The response to IL-12 is enhanced and is much more rapidwhen CD4 T cells are cultured in the presence of accessory cells or of exogenous IL-1, IL-2, or TNF-alpha. Using a three-step culture system, we next show that IL-12 induces the maturation of resting naive CD4T cells into cells producing both IL-2 and IFN-gamma but not IL-4 upon stimulation with PMA and ionomycin. Endogenously produced IFN-gamma plays a role in this IL-12-induced T cell maturation, as shown by the inhibitory effect of neutralizing IFN-gamma antibodies. Finally, we show that IL-12 supports the production of IFN-gamma during primary stimulation of neonatal T cells via the CD3/TCR complex by means of eitherimmobilized anti-CD3 mAb or superantigen-coated (Staphylococcus enterotoxin B) fixed L cell transfectants expressing HLA-DR. It is suggested that IL-12 is involved in the selection of Th1 type immune responses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 08:11:06