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Titolo:
DISTINCTION BETWEEN ARTIFACTUALLY SHRUNKEN AND TRULY DEGENERATED DARKNEURONS BY IN-SITU FIXATION WITH MICROWAVE IRRADIATION
Autore:
LOBERG EM; TORVIK A;
Indirizzi:
ULLEVAL UNIV HOSP,DEPT PATHOL,DIV NEUROPATHOL N-0407 OSLO NORWAY
Titolo Testata:
Neuropathology and applied neurobiology
fascicolo: 4, volume: 19, anno: 1993,
pagine: 359 - 363
SICI:
0305-1846(1993)19:4<359:DBASAT>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT CEREBRAL-CORTEX; LIGHT MICROSCOPIC RESPONSE; CONCUSSIVE HEAD-INJURY; HISTOLOGICAL FIXATION; BRAIN-LESIONS; ISCHEMIA; DAMAGE; DENDRITES; SOMATA; AXONS;
Keywords:
DARK NEURONS; MICROWAVE FIXATION;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
E.M. Loberg e A. Torvik, "DISTINCTION BETWEEN ARTIFACTUALLY SHRUNKEN AND TRULY DEGENERATED DARKNEURONS BY IN-SITU FIXATION WITH MICROWAVE IRRADIATION", Neuropathology and applied neurobiology, 19(4), 1993, pp. 359-363

Abstract

Dark, shrunken neurons frequently occur as artefacts in immersion fixed tissue. Perfusion fixation will prevent artefacts of this type. However, morphologically identical neurons have been described as truly degenerated cells in perfusion fixed brains in various pathological conditions. Since adequate perfusion is difficult to obtain in some pathological conditions, the question still remains whether the dark neurons found in some of these situations are true in vivo phenomena or artefacts caused by inadequate fixation. In the present study rat brains with cryogenic lesions were fixed in situ by microwave irradiation. With this method no artefactually changed dark neurons were observed in the normal parts of the brains. In the cryogenic lesions, however, a narrow rim of dark, shrunken neurons occurred adjacent to the normal cortex. This zone was identical to that observed in perfusion fixed tissue. Since inadequate fixation due to uneven perfusion of the damaged tissue is prevented with this method, we suggest that the neuronal changes represent true in vivo phenomena. Fixation with microwave irradiation can thus be used to differentiate between artefactually changed andtruly degenerated dark neurons in various pathological conditions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 09:50:33