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Titolo:
MULTIPLE CHANGES IN CHROMATIN STRUCTURE PRECEDE THE TRANSCRIPTIONAL ACTIVATION OF THE HUMAN GROWTH-HORMONE LOCUS IN PLACENTAL CELLS
Autore:
JIMENEZ G; FORD AM; ENVER T; BORONAT A;
Indirizzi:
UNIV BARCELONA,FAC QUIM,DEPT BIOQUIM & FISIOL,MARTI FRANQUES 1 E-08028 BARCELONA SPAIN UNIV BARCELONA,FAC QUIM,DEPT BIOQUIM & FISIOL,MARTI FRANQUES 1 E-08028 BARCELONA SPAIN INST CANC RES,CHESTER BEATTY LABS,LEUKAEMIA RES FUND CTR LONDON ENGLAND
Titolo Testata:
Molecular and cellular endocrinology
fascicolo: 1-2, volume: 96, anno: 1993,
pagine: 53 - 60
SICI:
0303-7207(1993)96:1-2<53:MCICSP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
TISSUE-SPECIFIC EXPRESSION; CHORIONIC SOMATOMAMMOTROPIN; GENE-CLUSTER; DNASE-I; HUMAN CHORIOCARCINOMA; HYPERSENSITIVE SITES; MESSENGER-RNA; C-MYC; EVOLUTION; LACTOGEN;
Keywords:
CHROMATIN; GROWTH HORMONE LOCUS; CHORIONIC SOMATOMAMMOTROPIN; DNASEI HYPERSENSITIVE SITES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
G. Jimenez et al., "MULTIPLE CHANGES IN CHROMATIN STRUCTURE PRECEDE THE TRANSCRIPTIONAL ACTIVATION OF THE HUMAN GROWTH-HORMONE LOCUS IN PLACENTAL CELLS", Molecular and cellular endocrinology, 96(1-2), 1993, pp. 53-60

Abstract

In addition to the growth hormone gene (hGH-N) itself, the human growth hormone (hGH) locus contains four related genes, namely hGH-V and hCS-L, -A and -B, which have appeared very recently in evolution and are specifically expressed in placenta. With the aim of identifying the regulatory elements responsible for this placental-specific expression, we have mapped the DNaseI hypersensitive sites present at the hGH gene cluster in a placental cell line (BeWo) that expresses the hGH-V and hCS genes. Our results reveal a complex pattern of hypersensitive sites distributed along the hGH locus, most of which appear to be cell type-specific. Thus, we have identified placental-specific hypersensitive sites within the first intron of the hGH-N and hGH-V genes, but notin the equivalent regions of the hCS genes. In addition, we have found several placental-specific hypersensitive sites downstream of the hCS-L and hCS-A genes, which might reflect the presence of enhancer elements similar to that located downstream of the hCS-B gene (Walker et al. (1990) J. Biol. Chem. 265, 12940). Comparison of BeWo cells with a placental cell line (JEG-3) which does not express the hGH-V and hCS genes revealed a very similar pattern of hypersensitive sites, suggesting that the sites detected are established before the onset of transcription. Our results indicate that the transition to an active hGH locus in placental cells requires multiple alterations in chromatin structure, and provide a framework for the molecular analysis of the regulatory elements and mechanisms mediating such processes.

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Documento generato il 04/12/20 alle ore 19:47:41