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Titolo:
4 YEAST SPLICEOSOMAL PROTEINS (PRP5, PRP9, PRP11, AND PRP21) INTERACTTO PROMOTE U2 SNRNP BINDING TO PREMESSENGER RNA
Autore:
RUBY SW; CHANG TH; ABELSON J;
Indirizzi:
UNIV NEW MEXICO,SCH MED,DEPT CELL BIOL ALBUQUERQUE NM 87131 UNIV NEW MEXICO,CTR CANC ALBUQUERQUE NM 87131 OHIO STATE UNIV,DEPT MOLEC GENET COLUMBUS OH 43210 CALTECH,DIV BIOL 147-75 PASADENA CA 91125
Titolo Testata:
Genes & development
fascicolo: 10, volume: 7, anno: 1993,
pagine: 1909 - 1925
SICI:
0890-9369(1993)7:10<1909:4YSP(P>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMALL NUCLEAR RIBONUCLEOPROTEIN; EUKARYOTIC TRANSLATION INITIATION; BASE-PAIRING INTERACTION; SACCHAROMYCES-CEREVISIAE; SPLICING INVITRO; ENDOPLASMIC-RETICULUM; STRUCTURAL ELEMENT; SYNTHETIC LETHAL; ACTIN MUTATIONS; U6 SNRNAS;
Keywords:
SPLICING; PREMESSENGER RNA; SPLICEOSOME; U2 SNRNP; PRP PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
98
Recensione:
Indirizzi per estratti:
Citazione:
S.W. Ruby et al., "4 YEAST SPLICEOSOMAL PROTEINS (PRP5, PRP9, PRP11, AND PRP21) INTERACTTO PROMOTE U2 SNRNP BINDING TO PREMESSENGER RNA", Genes & development, 7(10), 1993, pp. 1909-1925

Abstract

We have analyzed the functions of several pre-mRNA processing (PRP) proteins in yeast spliceosome formation. Here, we show that PRP5 (a DEAD box helicase-like protein), PRP9, and PRP11 are each required for the U2 snRNP to bind to the pre-spliceosome during spliceosome assembly in vitro. Genetic analyses of their functions suggest that they and another protein, PRP21, act concertedly and/or interact physically with each other and with the stem-loop IIa of U2 snRNA to bind U2 snRNP to the pre-mRNA. Biochemical complementation experiments also indicate that the PRP9 and PRP11 proteins interact. The PRP9 and PRP11 proteins may be functioning similarly in yeast and mammalian cells. The requirement for ATP and the helicase-like PRPS protein suggests that these factors might promote a conformational change (involving either the U1 orU2 snRNP) that is required for the association of U2 snRNP with the pre-mRNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 00:35:58