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Titolo:
RELEASE OF INTERLEUKIN-8, INTERLEUKIN-6, AND COLONY-STIMULATING FACTORS BY UPPER AIRWAY EPITHELIAL-CELLS - IMPLICATIONS FOR CYSTIC-FIBROSIS
Autore:
BEDARD M; MCCLURE CD; SCHILLER NL; FRANCOEUR C; CANTIN A; DENIS M;
Indirizzi:
UNIV SHERBROOKE,FAC MED,DEPT ANAT & CELLULAR BIOL,PULM RES UNIT,3001 12TH AVE N SHERBROOKE J1H 5N4 QUEBEC CANADA UNIV SHERBROOKE,FAC MED,DEPT ANAT & CELLULAR BIOL,PULM RES UNIT,3001 12TH AVE N SHERBROOKE J1H 5N4 QUEBEC CANADA UNIV CALIF RIVERSIDE,DEPT BIOL,DIV BIOMED SCI RIVERSIDE CA 92521
Titolo Testata:
American journal of respiratory cell and molecular biology
fascicolo: 4, volume: 9, anno: 1993,
pagine: 455 - 462
SICI:
1044-1549(1993)9:4<455:ROIIAC>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
PSEUDOMONAS-AERUGINOSA RHAMNOLIPIDS; TUMOR-NECROSIS-FACTOR; NEUTROPHIL ELASTASE; FIBRONECTIN FRAGMENTS; POTENTIAL MECHANISM; HUMAN-MONOCYTES; GROWTH-FACTOR; CYTOKINES; ACCUMULATION; LUNG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
M. Bedard et al., "RELEASE OF INTERLEUKIN-8, INTERLEUKIN-6, AND COLONY-STIMULATING FACTORS BY UPPER AIRWAY EPITHELIAL-CELLS - IMPLICATIONS FOR CYSTIC-FIBROSIS", American journal of respiratory cell and molecular biology, 9(4), 1993, pp. 455-462

Abstract

Cystic fibrosis (CF) is characterized by a dramatic neutrophil recruitment and repeated Pseudomonas infections in the lungs. To evaluate cytokine releasability by airway epithelial cells in the context of CF, we studied primary nasal epithelial cells isolated from the upper airways and continuous epithelial cell lines from normal and CF subjects. Relatively low levels of interleukin (IL)-8, IL-6, and granulocyte/macrophage colony-stimulating factor (GM-CSF) were produced spontaneouslyby primary epithelial cells (< 50 pg/10(6) cells) and higher levels of colony-stimulating factor-1 (CSF-1) (1 to 2 ng/10(6) cells). Cells were stimulated with substances that are likely to be present in the inflamed lungs of CF patients - namely, the proinflammatory monokines IL-1 and tumor necrosis factor-alpha (TNFalpha) as well as neutrophil elastase and bacterial products from Pseudomonas (mucoid exopolysaccharide [MEP] and rhamnolipids). Both IL-1 and TNFalpha induced a dose-dependent release of IL-6 (5 to 10 ng/10(6) cells) and GM-CSF (2 to 3 ng/10(6) cells) by primary epithelial cells from eight normal volunteers. The TNFalpha/IL-1-stimulated GM-CSF release was blocked by the addition of 1 muM dexamethasone, whereas basal CSF-1 release was unaffected. Neutrophil elastase.was a potent inducer of IL-8 and GM-CSF both in primary epithelial cells and in cell lines. Dexamethasone (1 muM) did not inhibit elastase-induced IL-8 release in either normal or CF epithelial cells. Rhamnolipids and MEP were found to stimulate the copious release of IL-8, GM-CSF, and IL-6 from epithelial cells, in a steroid-sensitive fashion. Epithelial cell lines from normal and CF subjects secreted very similar amounts of cytokines upon exposure to the various stimuli. We conclude from the above that: (1) proinflammatory cytokine release by epithelial cells is induced with leukocyte products and Pseudomonas bacterial products; (2) elastase induces IL-8 release in a manner unaffected by steroid treatment; (3) CSF-1 release occurs constitutively, and its spontaneous release may be related to a maintenance function for this cytokine; and (4) continuous epithelial cell lines obtained from CF subjects do not have an aberrant cytokine secretion profile. These results have implications in CF, where high levels of elastase, monokines, Pseudomonas rhamnolipids, and MEP are generated in the inflamed lungs of CF patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 07:27:15