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Titolo:
DETERMINATION OF MALONALDEHYDE BY COUPLED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY SPECTROFLUOROMETRY AFTER DERIVATIZATION WITH LUMINARIN-3
Autore:
TRAORE F; FARINOTTI R; MAHUZIER G;
Indirizzi:
UNIV PARIS 11,FAC SCI PHARMACEUT & BIOL,CHIM ANALYT METHODOL BIOANALYT LAB 2 F-92290 CHATENAY MALABRY FRANCE
Titolo Testata:
Journal of chromatography
fascicolo: 1, volume: 648, anno: 1993,
pagine: 111 - 118
Fonte:
ISI
Lingua:
ENG
Soggetto:
PEROXYOXALATE CHEMILUMINESCENCE DETECTION; THIOBARBITURIC ACID ADDUCT; FLUOROMETRIC METHOD; BIOLOGICAL-FLUIDS; MALONDIALDEHYDE; REAGENT; PLASMA; LIPOPEROXIDES; SEPARATION; ALDEHYDES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
F. Traore et al., "DETERMINATION OF MALONALDEHYDE BY COUPLED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY SPECTROFLUOROMETRY AFTER DERIVATIZATION WITH LUMINARIN-3", Journal of chromatography, 648(1), 1993, pp. 111-118

Abstract

A high-performance liquid chromatographic method with fluorescence detection is described for the specific determination of free malonaldehyde (MDA). Malonaldehyde was labelled with luminarin 3 in acetate buffer (pH 4.0) at room temperature in the dark. An aliquot of the reaction mixture was injected on to an octadecyl-bonded column using acetonitrile-imidazole buffer (30:70, v/v) as the mobile phase. The eluate wasmonitored with a fluorescence detector at 395 nm (excitation) and 500nm (emission). A linear calibration graph was established between 7.2and 90 ng/ml of MDA and the limit of quantification (LOQ) was lower than 7 ng/ml of MDA. The precision was characterized by R.S.D.s of 11% at 7.2 ng/ml and 2% at 90 ng/ml. The structure of the derivative was confirmed as the 5-hydroxy-2-pyrazoline form. UV absorbance and corrected fluorescence spectral data and quantum yields of the luminarin 3 derivative of malonaldehyde are presented.

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Documento generato il 16/07/20 alle ore 05:49:56