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Titolo:
OSMOPROTECTANTS IN HALOMONAS-ELONGATA - HIGH-AFFINITY BETAINE TRANSPORT-SYSTEM AND CHOLINE-BETAINE PATHWAY
Autore:
CANOVAS D; VARGAS C; CSONKA LN; VENTOSA A; NIETO JJ;
Indirizzi:
UNIV SEVILLE,FAC PHARM,DEPT MICROBIOL & PARASITOL E-41012 SEVILLE SPAIN UNIV SEVILLE,FAC PHARM,DEPT MICROBIOL & PARASITOL E-41012 SEVILLE SPAIN PURDUE UNIV,DEPT BIOL SCI W LAFAYETTE IN 47907
Titolo Testata:
Journal of bacteriology
fascicolo: 24, volume: 178, anno: 1996,
pagine: 7221 - 7226
SICI:
0021-9193(1996)178:24<7221:OIH-HB>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-DEPENDENT TRANSPORT; GLYCINE BETAINE; ESCHERICHIA-COLI; SALMONELLA-TYPHIMURIUM; HALOPHILIC EUBACTERIA; BACILLUS-SUBTILIS; OSMOREGULATION; PROLINE; ACCUMULATION; BIOSYNTHESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
D. Canovas et al., "OSMOPROTECTANTS IN HALOMONAS-ELONGATA - HIGH-AFFINITY BETAINE TRANSPORT-SYSTEM AND CHOLINE-BETAINE PATHWAY", Journal of bacteriology, 178(24), 1996, pp. 7221-7226

Abstract

The osmoregulatory pathways of the moderately halophilic bacterium Halomonas elongata DSM 3043 have been investigated, This strain grew optimally at 1.5 to 2 M NaCl in M63 glucose-defined medium, It required at least 0.5 M NaCl for growth, which is a higher concentration than that exhibited by the H. elongata type strain ATCC 33173, Externally provided betaine, choline, or choline-O-sulfate (but not proline, ectoine, or proline betaine) enhanced the growth of H. elongata on 3 M NaCl-glucose-M63 plates, demonstrating the utilization of these compounds asosmoprotectants. Moreover, betaine and choline stimulated the growth of H, elongata DSM 3043 over the entire range of salinity, although betaine was more effective than choline at salinities below and above the optimum, We found that H. elongata DSM 3043 has at least one high-affinity transport system for betaine (K-m = 3.06 mu M and V-max = 9.96 nmol of betaine min(-1) mg of protein(-1)), Competition assays demonstrated that proline betaine and ectoine, but not proline, choline, or choline-O-sulfate, are also transported by the betaine permease, Finally, thin-layer chromatography and C-13-nuclear magnetic resonance analysis showed that exogenous choline was taken up and transformed to betaine by H. elongata, demonstrating the existence of a choline-glycine betaine pathway in this moderately halophilic bacterium.

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Documento generato il 28/11/20 alle ore 04:35:39