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Titolo:
DEGRADATION OF SURFACTANT-ASSOCIATED PROTEIN-B (SP-B) DURING IN-VITROCONVERSION OF LARGE TO SMALL SURFACTANT AGGREGATES
Autore:
VELDHUIZEN RAW; INCHLEY K; HEARN SA; LEWIS JF; POSSMAYER F;
Indirizzi:
UNIV WESTERN ONTARIO,DEPT BIOCHEM LONDON N6A 5A5 ONTARIO CANADA UNIV WESTERN ONTARIO,DEPT PATHOL LONDON N6A 5A5 ONTARIO CANADA UNIV WESTERN ONTARIO,DEPT MED LONDON N6A 5A5 ONTARIO CANADA UNIV WESTERN ONTARIO,DEPT OBSTET & GYNAECOL LONDON N6A 5A5 ONTARIO CANADA UNIV WESTERN ONTARIO,MRC,FETAL & NEONATAL HLTH & DEV GRP LONDON N6A 5A5 ONTARIO CANADA
Titolo Testata:
Biochemical journal
, volume: 295, anno: 1993,
parte:, 1
pagine: 141 - 147
SICI:
0264-6021(1993)295:<141:DOSP(D>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESPIRATORY-DISTRESS SYNDROME; PULMONARY SURFACTANT; ALVEOLAR SURFACTANT; LUNG SURFACTANT; LAVAGE FLUID; METABOLISM; PHOSPHOLIPIDS; SUBFRACTIONS; SEPARATION; SUBTYPES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
R.A.W. Veldhuizen et al., "DEGRADATION OF SURFACTANT-ASSOCIATED PROTEIN-B (SP-B) DURING IN-VITROCONVERSION OF LARGE TO SMALL SURFACTANT AGGREGATES", Biochemical journal, 295, 1993, pp. 141-147

Abstract

Pulmonary surfactant obtained from lung lavages can be separated by differential centrifugation into two distinct subfractions known as large surfactant aggregates and small surfactant aggregates. The large-aggregate fraction is the precursor of the small-aggregate fraction. Theratio of the small non-surface-active to large surface-active surfactant aggregates increases after birth and in several types of lung injury. We have utilized an in vitro system, surface area cycling, to study the conversion of large into small aggregates. Small aggregates generated by surface area cycling were separated from large aggregates by centrifugation at 40000 g for 15 min rather than by the normal sucrosegradient centrifugation. This new separation method was validated by morphological studies. Surface-tension-reducing activity of total surfactant extracts. as measured with a pulsating-bubble surfactometer, was impaired after surface area cycling. This impairment was related to the generation of small aggregates. Immunoblot analysis of large and small aggregates separated by sucrose gradient centrifugation revealed the presence of detectable amounts of surfactant-associated protein B (SP-B) in large aggregates but not in small aggregates. SP-A was detectable in both large and small aggregates. PAGE of cycled and non-cycled surfactant showed a reduction in SP-B after surface area cycling. Weconclude that SP-B is degraded during the formation of small aggregates in vitro and that a change in surface area appears to be necessary for exposing SP-B to protease activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 15:44:59