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Titolo:
A UNIQUE, TERMINALLY GLUCOSYLATED OLIGOSACCHARIDE IS A COMMON FEATUREON LEISHMANIA CELL-SURFACES
Autore:
FUNK VA; THOMASOATES JE; KIELLAND SL; BATES PA; OLAFSON RW;
Indirizzi:
UNIV VICTORIA,DEPT BIOCHEM & MICROBIOL,POB 3055 VICTORIA BC V8W 3P6 CANADA UNIV UTRECHT,DEPT MASS SPECTROMETRY NL-3584 CA UTRECHT NETHERLANDS
Titolo Testata:
Molecular and biochemical parasitology
fascicolo: 1, volume: 84, anno: 1997,
pagine: 33 - 48
SICI:
0166-6851(1997)84:1<33:AUTGOI>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-BOUND OLIGOSACCHARIDES; N-GLYCOSIDASE-F; MEXICANA-AMAZONENSIS; LINKED OLIGOSACCHARIDES; TRYPANOSOMATID PROTOZOA; MAJOR PROMASTIGOTES; DONOVANI-CHAGASI; GLYCOSYLATION; GLYCOPROTEIN; LIPOPHOSPHOGLYCAN;
Keywords:
LEISHMANIA; OLIGOSACCHARIDE STRUCTURE; TERMINAL GLUCOSE; GP63;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
V.A. Funk et al., "A UNIQUE, TERMINALLY GLUCOSYLATED OLIGOSACCHARIDE IS A COMMON FEATUREON LEISHMANIA CELL-SURFACES", Molecular and biochemical parasitology, 84(1), 1997, pp. 33-48

Abstract

The structures of N-linked oligosaccharides from various Leishmania life-cycle stages and species have been investigated in order to elucidate differences which may be correlated with virulence or tissue tropisms. The structure of gp63 glycans from L. major log- and stationary-phase promastigotes were elucidated and compared with the total membrane associated oligosaccharides from five Leishmania spp. L. major gp63 glycans from promastigotes in either log or stationary phases of theirgrowth cycle were shown to have two neutral oligosaccharides having Bio-Gel P4 hydrodynamic volumes of 10.5 and 9.6 glucose units (GU). Sequential exoglycosidase digestion, fragmentation by acetolysis and methylation analysis of hydrazine released glycans; revealed the structureof G9.6 to be a biantennary oligomannose type, having the compositionMan(6)GlcNAc(2). These data were confirmed by structural analysis of gp63 oligosaccharides released by digestion with endo-beta-N-acetplglucosaminidase H (Endo-H) and N-glycanase F. The larger glycan was foundto be terminally glucosylated, having the composition GlcMan(6)GlcNAc(2). These oligosaccharides were found to occupy only two of the threepredicted N-linked glycosylation sites in the L. major gp63 molecule,at positions 300 and 407. On comparison with glycans from other Leishmania spp. and strains, these two oligosaccharides were consistently found to be the predominant promastigote structures. Following transformation to the amastigote stage, alterations in N-linked oligosaccharides appeared to be less consistent between species. L. m. mexicana amastigotes were found to display the same G10.5 and G9.6 glycans found onpromastigotes while L. donovani LV9 amastigotes were found to be devoid of N-linked glycans. Copyright (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 22:28:46