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Titolo:
REGULATION OF THE EXPRESSION OF MYCOBACTERIUM-AVIUM COMPLEX PROTEINS DIFFERS ACCORDING TO THE ENVIRONMENT WITHIN HOST-CELLS
Autore:
BERMUDEZ LE; PETROFSKY M;
Indirizzi:
KUZELL INST,CALIF PACIFIC MED CTR,RES INST,SUITE 305,2200 WEBSTER ST SAN FRANCISCO CA 94115
Titolo Testata:
Immunology and cell biology
fascicolo: 1, volume: 75, anno: 1997,
pagine: 35 - 40
SICI:
0818-9641(1997)75:1<35:ROTEOM>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
CULTURED HUMAN MACROPHAGES; EPITHELIAL-CELLS; TUBERCULOSIS; INFECTION; IDENTIFICATION; GROWTH; HT-29;
Keywords:
HT-29 EPITHELIAL CELLS; MACROPHAGES; MYCOBACTERIUM AVIUM; REGULATION OF PROTEIN SYNTHESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
L.E. Bermudez e M. Petrofsky, "REGULATION OF THE EXPRESSION OF MYCOBACTERIUM-AVIUM COMPLEX PROTEINS DIFFERS ACCORDING TO THE ENVIRONMENT WITHIN HOST-CELLS", Immunology and cell biology, 75(1), 1997, pp. 35-40

Abstract

Mycobacterium avium is an intracellular organism that can infect a number of cell types such as macrophages and epithelial cells. Each one of these cells represents a different environment that requires specific adaptation from the bacterium. The effect of uptake of M. avium andM. smegmatis by both human monocyte-derived macrophages in culture for 6 days, and HT-29 intestinal mucosal cell line on the bacterial synthesis of proteins were comparatively examined, Incorporation of [S-35]-methionine by the bacterium was measured at 30 min, 2, 4, and 24 h after infection. Effect of the uptake by cells was compared with bacteria not exposed to cells and bacteria submitted to different stresses such as heat, hyperosmolarity and acid pH. Uptake of M. avium by macrophages triggered the synthesis of 93, 65, 55 and 33 kDa, among other proteins in the bacteria. Between 2 and 4 h of exposure to the intracellular millieu, a number of additional proteins have their synthesis up-regulated such as 39, 31, 43, 42, 61 and 70 kDa. In contrast, uptake byepithelial cells is associated with the up-regulation of 27, 65, 71 and 72 kDa proteins, among others. In this case, exposure to the intracellular environment was associated with expression of a number of proteins that do not vary with time, The results of this study suggest that regulation of the expression of proteins in M. avium varies according to the mammalian cell bacteria they are exposed to, and is influenced by the stage of intracellular infection.

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Documento generato il 20/09/20 alle ore 04:59:29