Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
BINDING OF OUTER-MEMBRANE PREPARATIONS OF CAMPYLOBACTER-JEJUNI TO INT-457 CELL-MEMBRANES AND EXTRACELLULAR-MATRIX PROTEINS
Autore:
MOSER I; SCHRODER W;
Indirizzi:
FREE UNIV BERLIN,INST MIKROBIOL & TIERSEUCHEN,FABECKSTR 36A D-14195 BERLIN GERMANY FREE UNIV BERLIN,INST BIOCHEM,SFB 344 D-14195 BERLIN GERMANY
Titolo Testata:
Medical microbiology and immunology
fascicolo: 3, volume: 184, anno: 1995,
pagine: 147 - 153
SICI:
0300-8584(1995)184:3<147:BOOPOC>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPITHELIAL-CELLS; ESCHERICHIA-COLI; SURFACE-PROTEINS; EUKARYOTIC CELLS; INVITRO BINDING; ADHERENCE; IDENTIFICATION; INFECTION; ADHESION; COLONIZATION;
Keywords:
CAMPYLOBACTER JEJUNI; OUTER MEMBRANE; BINDING INT 407 CELLS; FIBRONECTIN; COLLAGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
I. Moser e W. Schroder, "BINDING OF OUTER-MEMBRANE PREPARATIONS OF CAMPYLOBACTER-JEJUNI TO INT-457 CELL-MEMBRANES AND EXTRACELLULAR-MATRIX PROTEINS", Medical microbiology and immunology, 184(3), 1995, pp. 147-153

Abstract

Binding of outer membrane (OM) preparations of the thermophilic Campylobacter species C. jejuni to epithelial cell membranes and extracellular matrix proteins were studied in an in vitro model system using enzyme-linked immunosorbent assay. The OM preparations exhibited significant binding to INT 407 intestinal cell membranes. The process of adhesion was modulated by enzymatic, chemical or immunological pretreatmentof the bacteria. Following oxidation of the lipopolysaccharide (LPS) with sodium meta-periodate, the OM preparations essentially retained their binding properties. After pretreatment with proteinase K, the OM preparations lost their binding capacity and the apparent molecular mass of the major OM protein shifted from 42 to 24 kDa. Preincubation ofC. jejuni bacteria with C. jejuni-specific antiserum reduced adhesionsignificantly; preincubation with LPS-specific monoclonal antibodies only to a minimal extent. The OM preparations also bound significantlyto the extracellular matrix proteins collagen and fibronectin; however, they bound virtually no bovine serum albumin or horse serum.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 07:28:46