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Titolo:
AUXIN-BINDING PROTEIN-1 DOES NOT BIND AUXIN WITHIN THE ENDOPLASMIC-RETICULUM DESPITE THIS BEING THE PREDOMINANT SUBCELLULAR LOCATION FOR THIS HORMONE-RECEPTOR
Autore:
TIAN HC; KLAMBT D; JONES AM;
Indirizzi:
UNIV N CAROLINA,DEPT BIOL,305 COKER HALL CHAPEL HILL NC 27599 UNIV N CAROLINA,DEPT BIOL CHAPEL HILL NC 27599 UNIV BONN,INST BOT W-5300 BONN GERMANY
Titolo Testata:
The Journal of biological chemistry
fascicolo: 45, volume: 270, anno: 1995,
pagine: 26962 - 26969
SICI:
0021-9258(1995)270:45<26962:APDNBA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ZEA-MAYS-L; HEYMANN NEPHRITIS ANTIGEN; ALPHA-2-MACROGLOBULIN RECEPTOR; MOLECULAR-CLONING; PLASMA-MEMBRANE; IMMUNOLOGICAL METHODS; COLEOPTILE MEMBRANES; MAIZE COLEOPTILES; SECRETORY PATHWAY; CORN COLEOPTILES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
61
Recensione:
Indirizzi per estratti:
Citazione:
H.C. Tian et al., "AUXIN-BINDING PROTEIN-1 DOES NOT BIND AUXIN WITHIN THE ENDOPLASMIC-RETICULUM DESPITE THIS BEING THE PREDOMINANT SUBCELLULAR LOCATION FOR THIS HORMONE-RECEPTOR", The Journal of biological chemistry, 270(45), 1995, pp. 26962-26969

Abstract

Auxin-binding protein 1 (ABP1) is a unique hormone receptor because it resides primarily in the lumen of the endoplasmic reticulum (ER); however, two lines of evidence presented here suggest that ABP1 does notbind auxin within the endoplasmic reticulum, despite its predominant location there, First, ABP1 cannot be photolabeled in intact cells that have accumulated the auxin and photolabeling reagent 5-[7-H-3]azidoindole-3-acetic acid, indicating either that auxin is excluded from theER and is not available for photolabeling to ABP1 or that binding conditions within the ER lumen are insufficient for photolabeling, Second, at the pH of the ER lumen, auxin binding to ABP1 is not detectable. The pH estimate of the ER lumen is based on an indirect assay, which indicates that the pH is closer to pH 7 than to the binding optimum of pH 5.5. These results indicate that ABP1 does not bind auxin within the ER and point to a site of action that is post-ER, The effect of auxin on its trafficking from the ER was tested in an animal expression system, ABP1 expressed at high levels in COS7 cells is efficiently retained in the ER lumen and is not secreted even in the presence of 190 muM indole-3-acetic acid, an auxin concentration that is 40 times abovethe K-d for indole-3-acetic acid binding to ABP1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 04:45:53