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Titolo:
PROSTATE TISSUE-SPECIFICITY OF THE PROSTATE-SPECIFIC ANTIGEN PROMOTERISOLATED FROM A PATIENT WITH PROSTATE-CANCER
Autore:
PANG S; TANEJA S; DARDASHITI K; COHAN P; KABOO R; SOKOLOFF M; TSO CL; DEKERNION JB; BELLDEGRUN AS;
Indirizzi:
UNIV CALIF LOS ANGELES,SCH MED,DEPT SURG,DIV UROL,10833 LE CONTE AVE LOS ANGELES CA 90095
Titolo Testata:
Human gene therapy
fascicolo: 11, volume: 6, anno: 1995,
pagine: 1417 - 1426
SICI:
1043-0342(1995)6:11<1417:PTOTPA>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-EXPRESSION; MESSENGER-RNA; ANDROGEN REGULATION; ACID-PHOSPHATASE; CELL-LINE; INDUCTION; KALLIKREIN; PROTEINS; ANTIBODY; SEQUENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
S. Pang et al., "PROSTATE TISSUE-SPECIFICITY OF THE PROSTATE-SPECIFIC ANTIGEN PROMOTERISOLATED FROM A PATIENT WITH PROSTATE-CANCER", Human gene therapy, 6(11), 1995, pp. 1417-1426

Abstract

We have cloned and characterized a 620-bp fragment of DNA that flanks5' of the prostate-specific antigen (PSA) gene from a prostate cancerpatient, Using DNA transfection, the efficacy of this putative promoter in regulating gene expression was quantitated in several prostate and nonprostate tissue cell lines, Our results demonstrated that the 620-dp DNA fragment actively drives gene expression in LNCaP, a PSA-producing prostate tumor cell. line, No promoter activity was detected in the non-PSA-producing prostate tumor lines, DU145 and PC-3, nor in a renal (R11) or breast (MCF-7) cancer cell line, Furthermore, the promoter activity could be regulated in vitro by androgen stimulation, Dihydrotestosterone (DHT) concentrations between 3 and 30 nM induced the highest promoter activity in the transfected LNCaP cells, which parallels the expression profile of the androgen receptor in LNCaP cells, In addition, our PSA promoter exhibited competitive inhibition of the endogenous genomic PSA promoter in transfected LNCaP cells, suggesting that prostate cell-specific DNA-binding proteins are required to activatethe PSA promoter, A cytomegalovirus IE1 promoter (CMV promoter) attached to the 5'-flanking region of the PSA promoter increased its potency four- to five-fold while retaining tissue specificity, Our data suggest that a strong tissue-specific negative regulatory element capable of overriding the nonspecific CMV promoter is present in the PSA promoter and confers its tissue specificity, The use of a highly specific promoter-driven gene vector will allow selective expression of therapeutic genes within PSA-producing prostate cancer cells, providing a unique strategy for prostate cancer gene therapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 06:48:44