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Titolo:
NEUTROPHILS ACTIVATED BY GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR EXPRESS RECEPTORS FOR INTERLEUKIN-3 WHICH MEDIATE CLASS-II EXPRESSION
Autore:
SMITH WB; GUIDA L; SUN QY; KORPELAINEN EI; VANDENHEUVEL C; GILLIS D; HAWRYLOWICZ CM; VADAS MA; LOPEZ AF;
Indirizzi:
ST MARYS HOSP,SCH MED,DEPT IMMUNOL LONDON W2 1PG ENGLAND HANSON CTR CANC RES,DIV HUMAN IMMUNOL ADELAIDE SA AUSTRALIA
Titolo Testata:
Blood
fascicolo: 10, volume: 86, anno: 1995,
pagine: 3938 - 3944
SICI:
0006-4971(1995)86:10<3938:NABGCF>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN POLYMORPHONUCLEAR NEUTROPHILS; HUMAN EOSINOPHILS; CELL-LINE; GM-CSF; IL-3; DIFFERENTIATION; INHIBITION; CYTOKINES; GAMMA; IGG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
W.B. Smith et al., "NEUTROPHILS ACTIVATED BY GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR EXPRESS RECEPTORS FOR INTERLEUKIN-3 WHICH MEDIATE CLASS-II EXPRESSION", Blood, 86(10), 1995, pp. 3938-3944

Abstract

Freshly isolated peripheral blood neutrophils, unlike monocytes and eosinophils, do not bind interleukin-3 (IL-3) or respond to IL-3. We show that neutrophils cultured for 24 hours in granulocyte-macrophage colony-stimulating factor (GM-CSF) express mRNA for the IL-3 receptor (R) alpha subunit, as shown by RNase protection assays, and IL-3R alpha chain protein, as shown by flow cytometric analysis using two different specific monoclonal antibodies. This effect was selective for GM-CSF, because granulocyte colony-stimulating factor, tumor necrosis factor-alpha, interferon-gamma, and IL-1 failed to induce the IL-3 receptor,Saturation binding curves with I-125- IL-3 and Scatchard transformation showed the presence of about 100 high-affinity and 4,000 low-affinity receptors. Because neutrophils have been shown to express human leukocyte antigen (HLA)-DR in response to GM-CSF, we examined the possibility that IL-3 could augment HLA-DR expression on GM-CSF-treated cells, We found that neutrophils incubated with 30 ng/mL IL-3 as well as 0.1 ng/mL GMCSF expressed a mean of 2.1-fold higher levels of HLA-DR than with GM-CSF alone (P<.005), confirming the signalling competence of the newly expressed IL-3R, This increase was seen even at maximal concentrations of GM-CSF and represents the first demonstration that GM-CSF and IL-3 can have an additive effect on mature human cells. The augmentation of HLA-DR by IL-3 was specific because it could be inhibited by a blocking anti-IL-3R antibody. Expression of class II molecules byneutrophils under these conditions may have significance for antigen presentation. These results provide further evidence for the role of GM-CSF as an amplification factor in inflammation by inducing neutrophil responsiveness to IL-3 produced by T cells or mast cells. (C) 1995 by The American Society of Hematology.

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Documento generato il 26/09/20 alle ore 05:45:22