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Titolo:
DETECTION AND FUNCTIONAL-CHARACTERIZATION OF P180, A NOVEL CELL-CYCLE-REGULATED YEAST TRANSCRIPTION FACTOR THAT BINDS RETINOBLASTOMA CONTROL ELEMENTS
Autore:
CUEVO RS; GARRETT S; HOROWITZ JM;
Indirizzi:
DUKE UNIV,MED CTR,DEPT MOL CANC BIOL,BOX 3686 DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT MOL CANC BIOL DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT MICROBIOL DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT MED DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT BIOCHEM DURHAM NC 27710
Titolo Testata:
The Journal of biological chemistry
fascicolo: 6, volume: 272, anno: 1997,
pagine: 3813 - 3822
SICI:
0021-9258(1997)272:6<3813:DAFOPA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-PRODUCT; SACCHAROMYCES-CEREVISIAE; DNA-BINDING; SP1-MEDIATED TRANSCRIPTION; SUSCEPTIBILITY GENE; NEGATIVE REGULATION; AMINO-TERMINUS; RB PROTEIN; EXPRESSION; PROMOTER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
61
Recensione:
Indirizzi per estratti:
Citazione:
R.S. Cuevo et al., "DETECTION AND FUNCTIONAL-CHARACTERIZATION OF P180, A NOVEL CELL-CYCLE-REGULATED YEAST TRANSCRIPTION FACTOR THAT BINDS RETINOBLASTOMA CONTROL ELEMENTS", The Journal of biological chemistry, 272(6), 1997, pp. 3813-3822

Abstract

In recent years it has become apparent that the cellular machinery governing cell cycle progression and transcription control are often homologous in yeast and mammalian cells, We and others have previously shown that the SP family of mammalian transcription factors regulates the transcription of a number of genes whose activities are governed by the product of the retinoblastoma (Rb) susceptibility gene, including c-FOS, c-MYC, TGF beta-1, IGF-II, and c-JUN, To determine whether a similar pathway of transcriptional regulation may function in yeast, we explored the possibility that transcription factors with nucleotide-binding specificities akin to those of the SP family are expressed in Saccharomyces cerevisiae and Schizosaccharomyces pombe, Here we report the detection of novel yeast proteins (S. cerevisiae, p180; S. pombe, p200) that specifically bind Rb-regulated promoter elements in vitro dependent on nucleotides that are also required for binding and trans-activation by SP family members in vivo. Our results indicate that the S. cerevisiae retinoblastoma control element-binding activity 1) requires zinc for association with DNA; 2) does not bind to SCB, MCB, or E2Fsites in vitro; 3) is cell cycle-regulated in a SWI6-independent fashion; and 4) maximally stimulates retinoblastoma control element-mediated transcription in early- to mid-S phase. Taken together, these data suggest that p180 may regulate the transcription of a subset of yeast genes whose expression is coincident with the onset and/or progressionof DNA replication.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:34:23