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Titolo:
SIMULTANEOUS DETECTION OF TDT-MEDIATED DUTP-BIOTIN NICK END-LABELING (TUNEL)-POSITIVE CELLS AND MULTIPLE IMMUNOHISTOCHEMICAL MARKERS IN SINGLE TISSUE-SECTIONS
Autore:
TORNUSCIOLO DRZ; SCHMIDT RE; ROTH KA;
Indirizzi:
WASHINGTON UNIV,SCH MED,DEPT PATHOL,BOX 8118,660 S EUCLID AVE ST LOUIS MO 63110
Titolo Testata:
BioTechniques
fascicolo: 5, volume: 19, anno: 1995,
pagine: 800 - 805
SICI:
0736-6205(1995)19:5<800:SDOTDN>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVOUS-SYSTEM; DEATH; COMBINATION; PHENOTYPE; ANTIGENS; GROWTH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
D.R.Z. Tornusciolo et al., "SIMULTANEOUS DETECTION OF TDT-MEDIATED DUTP-BIOTIN NICK END-LABELING (TUNEL)-POSITIVE CELLS AND MULTIPLE IMMUNOHISTOCHEMICAL MARKERS IN SINGLE TISSUE-SECTIONS", BioTechniques, 19(5), 1995, pp. 800-805

Abstract

We have modified the terminal deoxynucleotidyl transferase (TDT)-mediated dUTP-biotin nick end-labeling (TUNEL) method to permit the immunogold-silver intensification detection of apoptotic cells in tissue sections. Such sections can subsequently be processed for multi-labeling fluorescence microscopy, thus permitting the simultaneous detection ofsilver-positive apoptotic cells and three additional florescent signals. In this report, we combined TUNEL labeling with the fluorescent detection of bromodeoxyuridine-positive proliferating cells, growth-associated protein-43 (GAP-43) immunoreactive neurons and bisbenzimide-positive pyknotic cells in the embryonic mouse nervous system. This combination of stains allowed the simultaneous detection of proliferative, differentiated and apoptotic cells in the developing nervous system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 20:56:37