Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
HIGH-LEVEL IL-10 PRODUCTION BY MONOCLONAL ANTIBODY-STIMULATED HUMAN T-CELLS
Autore:
SCHWARZ M; MAJDIC O; KNAPP W; HOLTER W;
Indirizzi:
UNIV VIENNA,INST IMMUNOL,SFI,VIRCC,BORSCHKEF 8A A-1090 VIENNA AUSTRIA UNIV VIENNA,INST IMMUNOL,SFI,VIRCC A-1090 VIENNA AUSTRIA ST ANNA CHILDRENS HOSP,CHILDRENS CANC RES INST A-1090 VIENNA AUSTRIA
Titolo Testata:
Immunology
fascicolo: 3, volume: 86, anno: 1995,
pagine: 364 - 371
SICI:
0019-2805(1995)86:3<364:HIPBMA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
INHIBITS CYTOKINE PRODUCTION; GROWTH-FACTOR-BETA; INTERLEUKIN-10 IL-10; LYMPHOCYTES-B; ACTIVATION; MONOCYTES; CLONES; DIFFERENTIATION; PROLIFERATION; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
M. Schwarz et al., "HIGH-LEVEL IL-10 PRODUCTION BY MONOCLONAL ANTIBODY-STIMULATED HUMAN T-CELLS", Immunology, 86(3), 1995, pp. 364-371

Abstract

We investigated interleukin-10 (IL-10) production in freshly isolatedmononuclear cells and purified T cells in response to stimulation with monoclonal antibodies (mAb) recognizing CD3, CD2 and CD28, or with the bacterial products Staphylococcus aureus cells (SAG), staphylococcal enterotoxin (SEA) and lipopolysaccharide (LPS), IL-10 production wascompared with that of IL-2, IL-4 and interferon-gamma (IFN-gamma). Similar to the other cytokines, in peripheral blood mononuclear cells (PBMC) from adult donors the highest IL-10 levels were produced in response to CD2 plus CD28 stimulation, within 72-96 hr of stimulation. Levels of IL-10 in response to CD2 plus CD28 stimulation (1.9 +/- 1 ng/ml)exceeded those in response to SEA (0.25 +/- 0.16 ng/ml), SAC (0.43 +/- 0.42 ng/ml), or LPS (0.19 +/- 0.14 ng/ml) stimulation. With adult purified T cells, high levels of IL-10 and IL-4 were measured following CD3 plus CD28 stimulation, and the amounts of both T-helper type-2 (Th2) cytokines decreased following the addition of phorbol myristate acetate (PMA), whereas the synthesis of the Th1 cytokines IL-2 and IFN-gamma was enhanced. When PBMC were stimulated with a CD3 mAb and different other cytokines were added, strong enhancement of IL-10 production was seen upon the addition of IL-2, IL-4, IL-7, IL-12 and IFN-gamma, whereas inhibition was found with transforming growth factor-beta(1) (TGF-beta(1)). These data illustrate that in freshly isolated PBMC largeamounts of IL-10 can be induced rapidly by appropriate mAb stimulation, and that even in freshly isolated cells IL-4 and IL-10 show signs of parallel regulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 07:51:25