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Titolo:
EXPRESSION OF 18.6 CD23 ANTIGEN ON HUMAN LYMPHOID PROGENITOR-CELL LINES AND PHORBOL 12-MYRISTATE 13-ACETATE (PMA)-INDUCED MICROGLIA-SHAPED CELLS/
Autore:
OKUMURA A; KISHI H; TAGOH H; MINOWADA J; MURAGUCHI A;
Indirizzi:
TOYAMA MED & PHARMACEUT UNIV,FAC MED,DEPT IMMUNOL,SUGITANI 2630 TOYAMA 93001 JAPAN TOYAMA MED & PHARMACEUT UNIV,FAC MED,DEPT IMMUNOL TOYAMA 93001 JAPAN HAYASHIBARA BIOCHEM LABS INC,FUJISAKI CELL CTR FUJISAKI OKAYAMA 702 JAPAN
Titolo Testata:
Microbiology and immunology
fascicolo: 11, volume: 39, anno: 1995,
pagine: 879 - 890
SICI:
0385-5600(1995)39:11<879:EO1CAO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL LANGERHANS CELLS; DEPENDENT PROTEIN-KINASE; FC-EPSILON RECEPTOR; HUMAN LYMPHOCYTES-T; HUMAN B-CELL; IGE RECEPTORS; ALVEOLAR MACROPHAGES; EFFECTOR FUNCTION; CD23; EOSINOPHILS;
Keywords:
CD23; LYMPHOID PROGENITOR CELLS; MICROGLIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
A. Okumura et al., "EXPRESSION OF 18.6 CD23 ANTIGEN ON HUMAN LYMPHOID PROGENITOR-CELL LINES AND PHORBOL 12-MYRISTATE 13-ACETATE (PMA)-INDUCED MICROGLIA-SHAPED CELLS/", Microbiology and immunology, 39(11), 1995, pp. 879-890

Abstract

The nature of lymphoid progenitors and factor(s) determining commitment to either the T- or B-lymphocyte pathway are poorly understood in the human system. In this study, we generated a monoclonal antibody (MoAb), 18.6, that recognizes a cell surface antigen on a human lymphoid progenitor cell line (FL4.4), MoAb 18.6 reacted with lymphoid progenitor lines, B lymphoid cell lines, and myelomonocytic cell lines. It didnot react with any T cell or erythroid leukemic cell lines. Two colorFAGS analyses of normal lymphoid tissues showed that MoAb 18.6 reacted with a majority of CD20(+) mature B cells and a minority of CD64(+) monocytes, Molecules of 3 different sizes with MW of 34, 45, and 68 Kdwere precipitated with MoAb 18.6 from the lymphoid progenitor cell line, The 18.6 antigen was not expressed on a fetal liver-derived lymphoid progenitor-like cell line, FL1,4, which has the capacity to differentiate into microglia-shaped cells upon PMA-stimulation. Stimulation of FL1,4 cells with PMA induced expression of the 18.6 antigen within 24 hr and the microglia-shaped cells stained positively with MoAb 18.6. Finally, cloning of a cDNA that encoded the 18.6 antigen revealed that the 18.6 antigen is identical to the CD23 antigen, Taken together, these data suggest that the 18,6/CD23 antigen is expressed on lymphoid precursors at a very early stage of differentiation.

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Documento generato il 04/12/20 alle ore 06:19:10