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Titolo:
PRODUCTION OF ENDOTHELIUM-DERIVED FACTORS FROM SODDED EXPANDED POLYTETRAFLUOROETHYLENE GRAFTS
Autore:
LEWIS DA; LOWELL RC; CAMBRIA RA; ROCHE PC; GLOVICZKI P; MILLER VM;
Indirizzi:
MAYO CLIN & MAYO FDN,DEPT SURG,200 1ST ST SW,MED SCI BLDG 4-57 ROCHESTER MN 55905 MAYO CLIN & MAYO FDN,DEPT SURG ROCHESTER MN 55905 MAYO CLIN & MAYO FDN,DEPT PHYS & BIOPHYS ROCHESTER MN 55905
Titolo Testata:
Journal of vascular surgery
fascicolo: 1, volume: 25, anno: 1997,
pagine: 187 - 197
SICI:
0741-5214(1997)25:1<187:POEFFS>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; RELAXING FACTOR; BLOOD-FLOW; PLATELET-AGGREGATION; DEPENDENT RESPONSES; VASCULAR GRAFTS; GROWTH-FACTOR; NATRIURETIC PEPTIDE; AUTOGENOUS VEIN; NITRIC-OXIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
D.A. Lewis et al., "PRODUCTION OF ENDOTHELIUM-DERIVED FACTORS FROM SODDED EXPANDED POLYTETRAFLUOROETHYLENE GRAFTS", Journal of vascular surgery, 25(1), 1997, pp. 187-197

Abstract

Purpose: Experiments were designed to determine whether endothelium isolated from adipose tissue and sodded onto expanded polytetrafluoroethylene grafts release endothelium-derived vasoactive factors. Methods:Thin-walled expanded polytetrafluoroethylene grafts (6 mm internal diameter, 6 cm length, 30 mu m pore size), one sodded with autogenous endothelial cells, the other unsodded, were implanted bilaterally in carotid arteries of 30 male mongrel dogs. Dogs were treated with 325 mg aspirin daily. After 6 weeks grafts were excised and perfused in a bioassay system. Effluent from the grafts stimulated with either acetylcholine, thrombin, adenosine 5-diphosphate, or the calcium ionophore A23187 was superfused over rings of canine femoral arteries without endothelium contracted with phenylephrine. Effluent from the grafts was analyzed by radioimmunoassay for thromboxane B-2, 6-ketoprostaglandin F-1 alpha, endothelin-1, and C-type natriuretic peptide. Results: Ninety percent of the sodded grafts and 87% of the unsodded grafts were patentafter 6 weeks. Bioassay rings superfused with effluent from sodded grafts stimulated with acetylcholine relaxed significantly more than rings superfused with effluent from similarly stimulated unsodded grafts. Biochemical analysis of the effluent showed an increase in 6-keto prostaglandin F-1 alpha and C-type natriuretic peptide and a decrease in endothelin-1 and thromboxane B-2 release from the sodded compared withthe unsodded grafts. Scanning electron microscopy showed a continuouslayer of endothelial cells lining only the sodded grafts. Staining for alpha-actin and heavy-chain myosin showed a differentiated layer of smooth muscle below the endothelial layer on the sodded grafts. Finally, there was positive staining for C-type natriuretic peptide and endothelin-1 in the endothelium of the sodded grafts. Conclusions: These results indicate that endothelial cells of sodded expanded polytetrafluoroethylene grafts produce endothelium-derived vasoactive factors. In addition, receptor-coupled synthesis/release of these factors is retained in sodded endothelial cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:22:15