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Titolo:
A ROLE FOR NUCLEOSOME ASSEMBLY IN BOTH SILENCING AND ACTIVATION OF THE XENOPUS TR-BETA-A GENE BY THE THYROID-HORMONE RECEPTOR
Autore:
WONG JM; SHI YB; WOLFFE AP;
Indirizzi:
NICHHD,MOLEC MORPHOGENESIS UNIT BETHESDA MD 20892 NICHHD,MOLEC EMBRYOL LAB,MOLEC BIOL SECT BETHESDA MD 20892
Titolo Testata:
Genes & development
fascicolo: 21, volume: 9, anno: 1995,
pagine: 2696 - 2711
SICI:
0890-9369(1995)9:21<2696:ARFNAI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
RETINOID-X-RECEPTOR; TRANSCRIPTION FACTOR ACCESS; YEAST ALPHA-2 REPRESSOR; TUMOR VIRUS PROMOTER; RNA POLYMERASE-II; NUCLEAR RECEPTORS; PHO5 PROMOTER; AMPHIBIAN METAMORPHOSIS; POSITIONED NUCLEOSOMES; CHROMATIN STRUCTURE;
Keywords:
NUCLEOSOME ASSEMBLY; XENOPUS; THYROID HORMONE RECEPTOR GENE; TRANSCRIPTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
85
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Wong et al., "A ROLE FOR NUCLEOSOME ASSEMBLY IN BOTH SILENCING AND ACTIVATION OF THE XENOPUS TR-BETA-A GENE BY THE THYROID-HORMONE RECEPTOR", Genes & development, 9(21), 1995, pp. 2696-2711

Abstract

We have assembled the thyroid hormone-inducible promoter of the Xenopus thyroid hormone receptor (TR)beta A gene into chromatin using replication-coupled and -independent assembly pathways in vivo. We establish that heterodimers of TR and 9-cis retinoic acid receptors (RXR) can bind to their recognition sites within chromatin both in vivo and in vitro and alternately repress or activate transcription dependent on the absence or presence of thyroid hormone. Maximal transcriptional repression requires the presence of unliganded TR/RXR heterodimers during replication-coupled chromatin assembly. We demonstrate an increase in transcription directed by the TR beta A promoter of over two orders ofmagnitude in vivo, following the addition of thyroid hormone. This increase in transcription involves the relief of the repressed state that is established by the unliganded TR/RXR heterodimer during replication-coupled chromatin assembly. The association of thyroid hormone withthe chromatin-bound TR/RXR heterodimer leads to the disruption of local chromatin structure in a transcription-independent process. Thus, chromatin structure has multiple roles in the regulation of TR beta A gene expression in vivo: The TR/RXR heterodimer recognizes the responseelement within chromatin, TR/RXR makes use of the chromatin assembly process to silence transcription more efficiently, and TR/RXR directs the disruption of local chromatin structure in response to thyroid hormone.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:28:49