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Titolo:
SHORT-CHAIN LIPOPOLYSACCHARIDE MUTANTS OF SEROGROUP-B NEISSERIA-MENINGITIDIS OF POTENTIAL VALUE FOR PRODUCTION OF OUTER-MEMBRANE VESICLE VACCINES
Autore:
ANDERSEN SR; BJUNE G; LYNGBY J; BRYN K; JANTZEN E;
Indirizzi:
NATL INST PUBL HLTH,DEPT VACCINOL,GEITMYRSVEIEN 75 N-0462 OSLO NORWAY NATL INST PUBL HLTH,DEPT VACCINOL N-0462 OSLO NORWAY
Titolo Testata:
Microbial pathogenesis
fascicolo: 3, volume: 19, anno: 1995,
pagine: 159 - 168
SICI:
0882-4010(1995)19:3<159:SLMOSN>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
GONORRHOEAE LIPOOLIGOSACCHARIDES; OLIGOSACCHARIDE EPITOPES; CORE OLIGOSACCHARIDES; MONOCLONAL-ANTIBODIES; GAS-CHROMATOGRAPHY; PROTEINS; TRIFLUOROACETYLATION; ACID; POLYSACCHARIDE; METHANOLYSIS;
Keywords:
NEISSERIA MENINGITIDIS; MUTANT; PYOCIN; LIPOPOLYSACCHARIDES; OUTER MEMBRANE VESICLE VACCINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
55
Recensione:
Indirizzi per estratti:
Citazione:
S.R. Andersen et al., "SHORT-CHAIN LIPOPOLYSACCHARIDE MUTANTS OF SEROGROUP-B NEISSERIA-MENINGITIDIS OF POTENTIAL VALUE FOR PRODUCTION OF OUTER-MEMBRANE VESICLE VACCINES", Microbial pathogenesis, 19(3), 1995, pp. 159-168

Abstract

Four lipopolysaccharide (LPS) mutants (Mu-1 to Mu-4) were isolated after exposing Neisseria meningitidis strain 44/76 to pyocins from Pseudomonas aeruginosa. Parent strain LPS contained one major SDS-PAGE bandexpressing the immunotype determinants of L3,L3,7 and L3,7,9 and a minor band of higher mobility expressing the immunotype determinants of L8,L8a,L1,8,10 and L11. Each mutant LPS appeared as one SDS-PAGE band of higher mobility than the bands of the parent strain. None of these LPSs expressed the immunotype determinants of the parent strain, except Mu-4 LPS which reacted with the L11-specific MAb 4C4. Strain 44/76 LPS was found to contain galactose (Gal), glucose (Glc), heptose (Hep),glucosamine (GlcN), and 2-keto-3-deoxy-octulosonic acid (Kdo) in the molar ratios of 1.9:1.3:1.7:3.5:2.1. The corresponding ratios of the mutants were: Mu-4, 0:1.7:1.7:2.8:2.0; Mu-3, 0:0:1.7:2.4:1.6; Mu-2, 0:0:2.1 :1.8:2.0, Mu-1,0:0:0:1.8:1.9. Thus, all mutant LPSs lacked Gal and possessed less GlcN as compared to strain 44/76 LPS. Consequently, these mutants do not express the lacto-N-neo-tetraose (Gal1-4GlcN1-3Gal1-4Glc) commonly found as a part of meningococcal LPS and also on structures of human erythrocytes. These IFS mutants will be considered foruse in production of OMV vaccines without host-like antigens, which might favour induction of antibodies to more conserved epitopes of meningococcal LPS. (C) 1995 Academic Press Limited

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 17:37:48