Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
NITRIC-OXIDE DONORS INDUCE EXTRUSION OF CYCLIC-GMP FROM ISOLATED HUMAN BLOOD-PLATELETS BY A MECHANISM WHICH MAY BE MODULATED BY PROSTAGLANDINS
Autore:
RADZISZEWSKI W; CHOPRA M; ZEMBOWICZ A; GRYGLEWSKI R; IGNARRO LJ; CHAUDHURI G;
Indirizzi:
UNIV CALIF LOS ANGELES,SCH MED,DEPT PHARMACOL,10833 LE CONTE AVE LOS ANGELES CA 90024 UNIV CALIF LOS ANGELES,SCH MED,DEPT PHARMACOL LOS ANGELES CA 90024 UNIV CALIF LOS ANGELES,SCH MED,DEPT OBSTET & GYNECOL LOS ANGELES CA 90024 JAGIELLONIAN UNIV,SCH MED,DEPT PHARMACOL PL-31531 KRAKOW POLAND
Titolo Testata:
International journal of cardiology
fascicolo: 3, volume: 51, anno: 1995,
pagine: 211 - 220
SICI:
0167-5273(1995)51:3<211:NDIEOC>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
ATRIAL NATRIURETIC FACTOR; VASCULAR SMOOTH-MUSCLE; GUANOSINE 3',5'-MONOPHOSPHATE; STIMULATED ACCUMULATION; ENDOTHELIAL-CELLS; INHIBITION; EFFLUX; PLASMA; AMP; ERYTHROCYTES;
Keywords:
HUMAN BLOOD PLATELETS; CYCLIC GMP; NITRIC OXIDE DONORS; PDE INHIBITORS; PROBENECID; OUABAIN; PROSTAGLANDIN A(1) AND D-2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
W. Radziszewski et al., "NITRIC-OXIDE DONORS INDUCE EXTRUSION OF CYCLIC-GMP FROM ISOLATED HUMAN BLOOD-PLATELETS BY A MECHANISM WHICH MAY BE MODULATED BY PROSTAGLANDINS", International journal of cardiology, 51(3), 1995, pp. 211-220

Abstract

In the presence of 3-isobutyl-methylxanthine (IBMX), induction of cyclic 3',5'-guanosine monophosphate (GMP) production in human washed platelets (HWP) by nitric oxide donors (NOD) is followed by its accumulation in the surrounding medium in a time- and concentration-dependent manner. Thirty minutes incubation of HWP with 3-morphoiino-sydonimine (SIN-1, 10 mu M) at 37 degrees C resulted in a 4.6-fold increase of cyclic GMP in platelets, whereas in the extracellular medium the increasewas 17.6-fold, Similar results were obtained when other NOD such as S-nitroso-N-acetylpenicyllamine (SNAP) and 3-(2-metoxy-5-chlorophenyl)oxatriazol-5-imine (GEA 3184) and the selective phosphodiesterase inhibitor, zaprinast (M&B 22948, 10 mu M), were used. Probenecid (1-300 mu M), an inhibitor of organic anion transport, or ouabain (1-300 mu M), an inhibitor of Na+/K+ adenine triphosphate (ATP)-ase had no effect oncyclic GMP production or extrusion after stimulation with SIN-1. Significantly, prostaglandin A(1) (PGA(1)) and prostaglandin D-2 (PGD(2)) inhibited the efflux of cyclic GMP from platelets induced by SNAP (10 mu M) in a concentration-dependent fashion, with an IC50 of 63 +/- 16 and 143 +/- 17 mu M, respectively. These studies suggest that the extrusion of cyclic GMP from human platelets after activation of soluble guanylate cyclase by NOD may contribute to the control of cyclic GMP levels in platelets with potential physiological and therapeutic consequences.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 12:49:33