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Titolo:
DIFFERENTIAL FREEZING TOLERANCE OF RAT PANCREATIC-ISLETS DEPENDING ONTHEIR SIZE VARIATION
Autore:
SAKONJU I; TAURA Y; MAMBA K; SUZUKI T; TAKIMOTO K; NAKAICHI M; NAKAMA S;
Indirizzi:
YAMAGUCHI UNIV,DEPT VET SURG,1677-1 YOSHIDA YAMAGUCHI 753 JAPAN YAMAGUCHI UNIV,DEPT VET ANAT YAMAGUCHI 753 JAPAN YAMAGUCHI UNIV,UNITED GRAD SCH VET MED YAMAGUCHI 753 JAPAN YAMAGUCHI UNIV,FAC AGR YAMAGUCHI 753 JAPAN
Titolo Testata:
Journal of veterinary medical science
fascicolo: 5, volume: 57, anno: 1995,
pagine: 859 - 863
SICI:
0916-7250(1995)57:5<859:DFTORP>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-TEMPERATURE PRESERVATION; TRANSPLANTATION; DIMETHYLSULFOXIDE; LANGERHANS;
Keywords:
CRYOPRESERVATION; FREEZING TOLERANCE; IMAGE ANALYSIS; RAT PANCREATIC ISLET;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
I. Sakonju et al., "DIFFERENTIAL FREEZING TOLERANCE OF RAT PANCREATIC-ISLETS DEPENDING ONTHEIR SIZE VARIATION", Journal of veterinary medical science, 57(5), 1995, pp. 859-863

Abstract

We have investigated the freezing tolerance of rat pancreatic islets. Freshly isolated rat pancreatic islets were divided into three groupsbased on their longest diameter (small; 100-200 mu m, medium; 201-300mu m, large; >300 mu m. They were then cryopreserved at a slow cooling rate (-0.3 degrees C/min) in the presence of dimethyl sulfoxide (Me(2)SO) or ethylene glycol (EG). After storage at -196 degrees C for 1-4weeks, they were thawed and their ability to secrete insulin in response to fluctuations in glucose concentration was examined during threeconsecutive static incubations in vitro (1st; 2.8 mM, 2nd; 16.7 mM, 3rd; 2.8 mM). Morphological examination of the beta-granule population was determined by image analysis, and correlation with islets size wasanalyzed. The amount of insulin released from large-sized islets was significantly suppressed in EG (p<0.05) and Me(2)SO (p<0.01) groups compared to unfrozen islets. However, the mean volume of the large-sizedislets isolated from one rat accounted for 43.0% of the total volume. On the other hand, the amount of insulin released from small- and medium-sized islets did not differ from those of unfrozen islets, and their mean volumes were 13.2 and 43.8%, respectively. The percentage of cells with beta-granules was significantly correlated with size in bothEG (r= -0.52) and Me(2)SO (r= -0.35) groups, but no significant correlation was observed in the unfrozen islets groups. These findings suggest that large-sized islets are more susceptible to freezing injury than small- or medium-sized islets. Moreover, the volume distribution ofisolated islets indicated that it may be important to retain the ability of insulin secretion from the large-sized islets.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 07:21:49