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Titolo:
OKADAIC ACID REVERSIBLY INHIBITS NEURITE OUTGROWTH IN EMBRYONIC DORSAL-ROOT GANGLION NEURONS
Autore:
GIASSON BI; MUSHYNSKI WE;
Indirizzi:
MCGILL UNIV,DEPT BIOCHEM,3655 DRUMMOND ST MONTREAL PQ H3G 1Y6 CANADA MCGILL UNIV,DEPT BIOCHEM MONTREAL PQ H3G 1Y6 CANADA
Titolo Testata:
Journal of neurobiology
fascicolo: 2, volume: 32, anno: 1997,
pagine: 193 - 201
SICI:
0022-3034(1997)32:2<193:OARINO>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN PHOSPHATASE 2A; GROWTH; PHOSPHORYLATION; DYNAMICS; CELLS;
Keywords:
DORSAL ROOT GANGLION; NEURITE OUTGROWTH; OKADAIC ACID; PROTEIN PHOSPHATASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
B.I. Giasson e W.E. Mushynski, "OKADAIC ACID REVERSIBLY INHIBITS NEURITE OUTGROWTH IN EMBRYONIC DORSAL-ROOT GANGLION NEURONS", Journal of neurobiology, 32(2), 1997, pp. 193-201

Abstract

The aim of this study was to assess the effects of low concentrationsof okadaic acid (OA) on neurite outgrowth and cellular integrity in cultures of dissociated dorsal root ganglion (DRG) neurons. The complete and fully reversible arrest of neurite outgrowth was achieved at 1 nM OA, thus ruling out the involvement of protein phosphatase 1 in the observed inhibitory effect. OA at 0.5 nM did not completely block neurite outgrowth, although it reduced the rate of growth by about one third. Protein phosphorylation and the integrity of microtubules and neurofilaments in neuron-enriched cultures were unaffected by 1 nM OA. Therate of synthesis of the low-molecular-weight neurofilament subunit (NFL) was also unchanged by OA treatment. Antimitotic agents used to eliminate proliferating cells did not alter the rate of neurite elongation, Since 1 nM OA does not suffice to inhibit neuronal protein phosphatase 2A fully, owing to the high concentration of this enzyme in neurons, we propose that the inhibitor is affecting a neuronal compartment that contains low levels of the phosphatase. This putative compartmentis likely to be located in neurites, which were shown to contain levels of protein phosphatase 2A that were two- to threefold lower than inneuronal perikarya. (C) 1997 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 14:22:00